Immunopotentiation and anti-tumor activity of carboxymethylated-sulfated β-(1→3)-D-glucan from Poria cocos
Immunopotentiation and anti-tumor activity of carboxymethylated-sulfated β-(1→3)-D-glucan from Poria cocos
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DOI：
10.1016/j.intimp.2010.01.002
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发表时间：
2010-04-01
影响因子：
5.6
通讯作者：
Cheung, Peter Chi Keung
中科院分区：
文献类型：
--
作者：
Chen, Xiaoyu;Zhang, Lina;Cheung, Peter Chi Keung
以茯苓为原料，合成了一种羧基甲基磺化的(1 - >3)- β - d-葡聚糖（PCS3-II）衍生物，编码为CS-PCS3-II。红外光谱（IR）和碳-13核磁共振谱（C-13 NMR）结果表明，CS-PCS3-II含有羧基甲基和硫酸盐基团，取代度（DS）分别为1.05和0.36。采用尺寸排除色谱（SEC）和激光散射（LLS）相结合的方法，在25℃0.15 M NaCl溶液中建立了CS-PCS3-II的旋转半径(（1/2)(z)）与分子量（M-w）的依赖关系为< S-2 >(1/2)(z) = 6.92 × 10(-2)M(w)(0.59)，表明CS-PCS3-II是一个延伸的柔性链。CS-PCS3-II对BALB/c小鼠180肉瘤的抑制率明显高于PCS3-II。经CS-PCS3-II处理的肿瘤细胞组织学检查有坏死和凋亡征象。假设PCS3-II中引入羧甲基和硫酸盐基团，增加了PCS3-II通过氢结合和静电吸引与免疫细胞受体接触的可能性，从而产生更强的免疫反应，从而抑制肿瘤细胞的增殖。此外，白细胞指数、胸腺指数、脾脏指数、溶血活性以及脾脏抗体产生和延迟型超敏反应（DTH）均显著升高，提示CS-PCS3-II可显著增强小鼠免疫增强作用。(C) 2010 Elsevier B.V.版权所有
A carboxymethylated-sulfated derivative of (1 -> 3)-beta-D-glucan (PCS3-II) extracted from Poria cocos was synthesized and coded as CS-PCS3-II. Results of infrared (IR) and Carbon-13 nuclear magnetic resonance spectroscopy (C-13 NMR) indicated that CS-PCS3-II contained carboxymethyl and sulfate groups with a degree of substitution (DS) of 1.05 and 0.36 respectively. By using size exclusion chromatography (SEC) combined with laser light scatting (LLS), the dependence of radius of gyration ((1/2)(z)) on the molecular weight (M-w) for CS-PCS3-II was established as < S-2 >(1/2)(z) = 6.92 x 10(-2)M(w)(0.59) in 0.15 M NaCl solution at 25 degrees C, suggesting that CS-PCS3-II existed as an extended flexible chain. CS-PCS3-II exhibited significantly higher inhibition ratio to Sarcoma 180 tumor in BALB/c mice than PCS3-II. Histological examination of tumor cells treated with CS-PCS3-II had signs of necrosis and apoptosis. It is postulated that introduction of the carboxymethyl and sulfate groups to PCS3-II increased its possible contact with the receptors of immune cells through hydrogen binding and electrostatic attraction, leading to a stronger immunological responses that resulted in inhibition of tumor cell proliferation. Moreover, there were significant increases in phagocyte and thymus indexes, spleen index, hemolytic activity as well as spleen antibody production and delayed type hypersensitivity (DTH), suggesting that CS-PCS3-II could significantly enhance immunpotentiation in mice. (C) 2010 Elsevier B.V. All rights reserved.