Alterations of antibody binding properties and active-site dimensions in the primary and secondary immune response.
Alterations of antibody binding properties and active-site dimensions in the primary and secondary immune response.
复制标题
初次和二次免疫反应中抗体结合特性和活性位点尺寸的改变。
DOI：
10.1021/bi00796a016
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发表时间：
1971
期刊：
影响因子：
2.9
通讯作者：
J. R. Little
中科院分区：
文献类型：
--
作者：
J. Hsia;J. R. Little
J. C. Hsiaf和J. Russell Little[摘要]；利用电子自旋共振光谱和荧光猝灭技术，对两种自旋标记的2,4,6 -三硝基苯（TNP）配体的活性位点进行了研究。用3-氨基或3-甲基氨基- 2,2,5,5 -四甲基吡咯烷硝基取代同源TNP基团会对抗体-半抗原复合物的形成产生空间扰动。在一次或二次免疫反应中产生的抗体结合位点的结构差异是从程度上推断出来的
J. C. Hsiaf and J. Russell Little J abstract; Rabbit antibodies specific for the 2, 4, 6-trinitrophenyl (TNP) determinant group were obtained from the primary and the secondary response and their active sites were studied with two spin-labeled 2, 4, 6-trinitrophenyl ligands using electron spin resonance spectroscopy and fluorescence quenching. The substitution of the homologous TNP group with a 3-amino-or 3-methylamino-2, 2, 5, 5-tetramethylpyrrolidine nitroxide introduced steric perturbations to the antibody-hapten complex formation. Differences in structure of the combining sites of antibodies produced during the primary or secondary immune response were inferred from theextent