Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage
Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage
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DOI：
10.1002/jor.1100170119
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发表时间：
1999-01-01
影响因子：
2.8
通讯作者：
Freed, LE
中科院分区：
文献类型：
--
作者：
Vunjak-Novakovic, G;Martin, I;Freed, LE
软骨构建体已经在体外使用分离的细胞、可生物降解的聚合物支架和生物反应器生长。在目前的工作中，工程化软骨结构的组成和力学性能之间的关系进行了研究，通过培养小牛关节软骨细胞纤维聚乙醇酸支架（5毫米直径，2毫米厚，97%的多孔）在三个不同的环境：静态烧瓶，混合烧瓶，旋转血管。培养6周后，径向限制静态和动态压缩的结构的组成、形态和机械功能都取决于体外培养的条件。静态培养产生了小而脆弱的结构，而混合烧瓶中的湍流产生了具有纤维外囊的结构;两种环境都导致了机械性能差的结构。在旋转血管中的动态层流场中自由悬浮培养的结构是最大的，含有连续的软骨样细胞外基质，具有最高分数的糖胺聚糖和胶原蛋白，并且具有最好的机械性能。平衡模量，透水性，动态刚度，和流动电位与糖胺聚糖，胶原蛋白和水的湿重分数。这些研究结果表明，在组织培养生物反应器的流体动力学条件可以调节工程软骨的组成，形态，机械性能和机电功能。
Cartilaginous constructs have been grown in vitro with use of isolated cells, biodegradable polymer scaffolds, and bioreactors. In the present work, the relationships between the composition and mechanical properties of engineered cartilage constructs were studied by culturing bovine calf articular chondrocytes on fibrous polyglycolic acid scaffolds (5 mm in diameter, 2-mm thick, and 97% porous) in three different environments: static flasks, mixed flasks, and rotating vessels. After 6 weeks of cultivation, the composition, morphology, and mechanical function of the constructs in radially confined static and dynamic compression all depended on the conditions of in vitro cultivation. Static culture yielded small and fragile constructs, while turbulent flow in mixed flasks yielded constructs with fibrous outer capsules; both environments resulted in constructs with poor mechanical properties. The constructs that were cultured freely suspended in a dynamic laminar flow field in rotating vessels were the largest, contained continuous cartilage-like extracellular matrices with the highest fractions of glycosaminoglycan and collagen, and had the best mechanical properties. The equilibrium modulus, hydraulic permeability, dynamic stiffness, and streaming potential correlated with the wet-weight fractions of glycosaminoglycan, collagen, and water. These findings suggest that the hydrodynamic conditions in tissue-culture bioreactors can modulate the composition, morphology, mechanical properties, and electromechanical function of engineered cartilage.