Rapid prototyping of tissue-engineering constructs, using photopolymerizable hydrogels and stereolithography
Rapid prototyping of tissue-engineering constructs, using photopolymerizable hydrogels and stereolithography
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DOI：
10.1089/ten.2004.10.1316
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发表时间：
2004-09-01
影响因子：
--
通讯作者：
Boland, T
中科院分区：
文献类型：
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作者：
Dhariwala, B;Hunt, E;Boland, T
组织工程学最重要的方面之一是支架的设计，为新组织提供机械强度和营养。对于定制的组织工程，必须能够制造各种几何形状的三维支架，以修复由事故，手术或出生引起的缺陷。快速成型或固体自由成型制造（SFF）技术在设计三维定制支架方面有很大的希望，但传统的细胞接种技术可能无法为更大的结构提供足够的细胞质量。本文提出了一种新的尝试，以制造三维支架，使用水凝胶结合细胞封装，以制造高密度的组织结构。一个商业上可用的立体光刻技术被施加到使用聚（环氧乙烷）和聚（乙二醇）二甲基丙烯酸酯光聚合水凝胶制造支架。力学表征表明，结构在弹性方面与软组织相当。实现了高细胞活力并制造了高密度构建体。
One of the most important aspects of tissue engineering is the design of the scaffold providing the mechanical strength and access to nutrients for the new tissue. For customized tissue engineering, it is essential to be able to fabricate three-dimensional scaffolds of various geometric shapes, in order to repair defects caused by accidents, surgery, or birth. Rapid prototyping or solid free-form fabrication (SFF) techniques hold great promise for designing three-dimensional customized scaffolds, yet traditional cell-seeding techniques may not provide enough cell mass for larger constructs. This article presents a novel attempt to fabricate three-dimensional scaffolds, using hydrogels combined with cell encapsulation to fabricate high-density tissue constructs. A commercially available stereolithography technique was applied to fabricate scaffolds using poly( ethylene oxide) and poly( ethylene glycol) dimethacrylate photopolymerizable hydrogels. Mechanical characterization shows the constructs to be comparable with soft tissues in terms of elasticity. High cell viability was achieved and high-density constructs fabricated.