Dinoflagellates alter their carbon and nutrient metabolic strategies across environmental gradients in the central Pacific Ocean
Dinoflagellates alter their carbon and nutrient metabolic strategies across environmental gradients in the central Pacific Ocean
复制标题
在太平洋中部的环境梯度中，鞭毛藻改变了它们的碳和营养代谢策略
DOI：
10.1038/s41564-020-00814-7
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发表时间：
2021-01-04
影响因子：
28.3
通讯作者：
Saito, Mak A.
中科院分区：
文献类型：
--
作者：
Cohen, Natalie R.;McIlvin, Matthew R.;Saito, Mak A.
通过对中太平洋4,600 km样带的地球化学和多组学分析，揭示了甲藻在生态系统和海洋地球化学过程中的重要作用。海洋微真核生物通过向高营养级转移能量和垂直碳运输，在海洋地球化学循环中发挥着重要作用。尽管它们具有全球重要性，但微真核生物的生理学、营养代谢和对近海生态系统碳循环的贡献的特征很差。在这里，我们观察到的患病率的甲藻沿着4,600公里的纬向样带延伸横跨太平洋中部，在那里贫营养环流满足赤道涌升沃茨丰富的常量营养素，但低溶解铁。一个综合的多组学和地球化学分析提供了一个窗口，甲藻代谢的横断面，表明一个连续的分类甲藻群落，转移其功能转录组和蛋白质组，因为它从真光层延伸到中层。在真光沃茨，多组学数据表明，营养模式的组合被利用，而中层代谢的特点是细胞骨架投资和营养循环。在营养物质代谢的重排是明显的，在整个梯度的可变氮和铁制度，没有相关的变化，在社区组合。总甲藻蛋白质缩放颗粒碳出口，两者都在赤道沃茨升高，表明甲藻丰度和总碳通量之间的联系。甲藻采用多种代谢策略，使广泛占领中太平洋生态系统，并发挥双重作用，通过光合作用固定在真光层和中远洋区的光合作用的碳转化。
A combined geochemical and multi-omics analysis across a 4,600-km transect in the central Pacific Ocean reveals that dinoflagellates play a previously unrecognized role in ecosystem and biogeochemical processes.Marine microeukaryotes play a fundamental role in biogeochemical cycling through the transfer of energy to higher trophic levels and vertical carbon transport. Despite their global importance, microeukaryote physiology, nutrient metabolism and contributions to carbon cycling across offshore ecosystems are poorly characterized. Here, we observed the prevalence of dinoflagellates along a 4,600-km meridional transect extending across the central Pacific Ocean, where oligotrophic gyres meet equatorial upwelling waters rich in macronutrients yet low in dissolved iron. A combined multi-omics and geochemical analysis provided a window into dinoflagellate metabolism across the transect, indicating a continuous taxonomic dinoflagellate community that shifted its functional transcriptome and proteome as it extended from the euphotic to the mesopelagic zone. In euphotic waters, multi-omics data suggested that a combination of trophic modes were utilized, while mesopelagic metabolism was marked by cytoskeletal investments and nutrient recycling. Rearrangement in nutrient metabolism was evident in response to variable nitrogen and iron regimes across the gradient, with no associated change in community assemblage. Total dinoflagellate proteins scaled with particulate carbon export, with both elevated in equatorial waters, suggesting a link between dinoflagellate abundance and total carbon flux. Dinoflagellates employ numerous metabolic strategies that enable broad occupation of central Pacific ecosystems and play a dual role in carbon transformation through both photosynthetic fixation in the euphotic zone and remineralization in the mesopelagic zone.