Green synthesis of silver nanoparticles using aqueous extract of saffron ( Crocus sativus L.) wastages and its antibacterial activity against six bacteria
Green synthesis of silver nanoparticles using aqueous extract of saffron ( Crocus sativus L.) wastages and its antibacterial activity against six bacteria
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DOI：
10.1016/j.apjtb.2016.12.014
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发表时间：
2017-03-01
影响因子：
1.7
通讯作者：
Namaei, Mohmmad Hasan
中科院分区：
文献类型：
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作者：
Bagherzade, Ghodsieh;Tavakoli, Maryam Manzari;Namaei, Mohmmad Hasan
目的：利用番红花提取物制备银纳米颗粒（AgNPs）。方法：本文采用绿色方法，在不添加任何化学稳定剂和还原剂的情况下，利用紫茎泽兰废弃物水提液制备银纳米粒子。结果：AgNPs的紫外-可见吸收光谱在450 nm处有一吸收峰，这是由于表面等离子体振动的激发。傅立叶透射红外光谱表明，纳米粒子被植物次生代谢产物所覆盖。X射线衍射分析也表明，合成的纳米粒子的尺寸范围为12-20 nm。透射电子显微镜图像示出了具有球形形状和15 nm的平均尺寸的AgNP。结论：生物合成的AgNPs对大肠杆菌、铜绿假单胞菌、肺炎克雷伯菌、福氏志贺菌和枯草芽孢杆菌均有较好的抑菌效果，可应用于生物医学领域。
Objective: To synthesis silver nanoparticles (AgNPs) by using extract of saffron (Crocus sativus L.) wastages and to test their antibacterial activity against six bacteria.Methods: In this paper, the synthesis of AgNPs using aqueous extract of saffron wastage as a green method without any chemical stabilizer and reducer is demonstrated. The synthesized AgNPs were determined by UV-vis spectrum, high resolution transmission electron microscopy, X-ray diffraction, and Fourier transmission infrared spectroscopy analysis.Results: UV-vis spectrum showed a peak at 450 nm due to excitation of surface plasmon vibrations. Fourier transmission infrared spectroscopy showed that nanoparticles were capped with plant secondary metabolites. X-ray diffraction analysis also demonstrated that the size range of the synthesized nanoparticles was 12-20 nm. Transmission electron microscope image illustrated AgNPs with spherical shape and an average size of 15 nm. The result of antibacterial activities showed that the biosynthesized AgNPs had an inhibiting activity against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Shigella flexneri and Bacillus subtilis.Conclusions: The biosynthesized AgNPs showed significant antibacterial effect against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Shigella flexneri and Bacillus subtilis, so, it can be used in biomedical applications.