Cytokine gene expression of endothelial cells infected with Trypanosoma cruzi.
Cytokine gene expression of endothelial cells infected with Trypanosoma cruzi.
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感染克氏锥虫的内皮细胞的细胞因子基因表达。
DOI：
10.1093/infdis/166.3.598
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Wittner,M
中科院分区：
文献类型：
--
作者：
Tanowitz,HB;Gumprecht,JP;Spurr,D;Calderon,TM;Ventura,MC;Raventos-Suarez,C;Kellie,S;Factor,SM;Hatcher,VB;Wittner,M
冠状动脉微血管痉挛和血小板高反应性与恰加斯心肌病的发病机制有关。为了进一步阐明微脉管系统在这种疾病中的作用，检查了人脐静脉内皮细胞因克氏锥虫感染而引起的细胞因子基因表达的变化。内皮细胞总 RNA 的 Northern 印迹分析表明，白细胞介素 (IL)-1β、IL-6 和集落刺激因子 1 (CSF-1) mRNA 表达在未感染的细胞中不存在或很少，但在感染的细胞中显着增加。 c-sismRNA水平随着感染时间的增加而降低。原位杂交研究还表明，单个感染细胞中 IL-6 mRNA 水平较高。在感染内皮细胞的上清液中检测到显着水平的IL-6和IL-1β蛋白。急性感染个体的血清含有高水平的 IL-6 蛋白，表明血管内皮分泌的细胞因子在恰加斯心肌病的发病机制中具有潜在的重要性。
Coronary microvascular spasm and platelet hyperreactivity have been implicated in the pathogenesis of Chagas' cardiomyopathy. To clarify further the role of the microvasculature in this disease, alterations in cytokine gene expression due toTrypanosoma cruziinfection of human umbilical vein endothelial cells were examined. Northern blot analysis of total RNA from endothelial cells demonstrated that interleukin (IL)-1β, IL-6, and colony-stimulating factor 1 (CSF-1) mRNA expression was absent or minimal in uninfected cells but significantly increased in infected cells. c-sismRNA levels diminished with increased time of infection. In situ hybridization studies also demonstrated high levels of IL-6 mRNA in individual infected cells. Significant levels of IL-6 and IL-1βprotein were detected in the supernatants of infected endothelial cells. The serum of an acutely infected individual contained high levels of IL-6 protein, suggesting the potential importance of cytokines secreted by the vascular endothelium in the pathogenesis of Chagas' cardiomyopathy.