C. elegans Rab GTPase 2 is required for the degradation of apoptotic cells
C. elegans Rab GTPase 2 is required for the degradation of apoptotic cells
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DOI：
10.1242/dev.016063
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发表时间：
2008-03-15
期刊：
影响因子：
4.6
通讯作者：
Wang, Xiaochen
中科院分区：
文献类型：
--
作者：
Lu, Qun;Zhang, Yan;Wang, Xiaochen
在凋亡过程中，垂死的细胞激活一种内在机制，迅速使自己死亡。凋亡细胞尸体然后被邻近细胞或专职吞噬细胞识别并移除。死亡细胞如何在内化后降解还知之甚少。在这里，我们报告的识别和表征的UNC-108，秀丽隐杆线虫同源物的人类Rab GTd 2，作为一种新的组件参与凋亡细胞的降解。UNC-108在吞噬细胞中表达和起作用，并且可能影响细胞尸体的降解而不是内化。与其他Rab GTP酶类似，unc-108也影响内吞作用，作用于从早期内体到晚期内体和晚期内体到溶酶体的内体运输。RAB 108与RAB-5、RAB-7和LMP-1共定位于吞噬体，并促进细胞尸体降解，可能是通过介导吞噬体成熟。
During apoptosis, the dying cell activates an intrinsic mechanism that quickly dismantles itself. The apoptotic cell corpses are then recognized and removed by neighboring cells or professional phagocytes. How dying cells are degraded after internalization is poorly understood. Here, we report the identification and characterization of unc-108, the Caenorhabditis elegans homolog of the human Rab GTPase 2, as a novel component involved in the degradation of apoptotic cells. unc-108 is expressed and functions in the engulfing cells and is likely to affect the degradation rather than the internalization of cell corpses. Similar to other Rab GTPases, unc-108 also affects endocytosis, acting in the endosomal trafficking from early to late endosome and late endosome to lysosome. UNC-108 co-localizes with RAB-5, RAB-7 and LMP-1 to the phagosome and promotes cell corpse degradation, possibly by mediating phagosome maturation.