INTEROBSERVER AND INTRAOBSERVER VARIATION IN BONE HISTOMORPHOMETRY
INTEROBSERVER AND INTRAOBSERVER VARIATION IN BONE HISTOMORPHOMETRY
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DOI：
10.1007/bf02556831
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发表时间：
1986-02-01
影响因子：
4.2
通讯作者：
STELLON, AJ
中科院分区：
文献类型：
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作者：
COMPSTON, JE;VEDI, S;STELLON, AJ
对20例正常人髂骨活检标本进行了组织形态计量学指标的观察。使用目镜分度尺和目镜千分尺进行定量。两名观察员检查了相同的章节，方法是相同的。观察者内部的变异也被评估。观察者间在骨小梁总体积、类骨质体积和表面积、双加单和双四环素标记表面以及平均类骨质骨缝宽度的测量上存在显著差异。由于观察者间的差异，类骨质表面和体积、总吸收表面和平均类骨质骨缝宽度的百分比差异最大。两个观察者的观察者内差异都很小。我们的结论是，即使在切片准备和方法相同的情况下，一些组织形态计量学指数的测量也可能在观察者之间发生很大的差异。在根据来自其他观察者的严格定义的对照数据诊断代谢性骨病时应谨慎，特别是当这些数据是从观察者间变异的影响可能因方法差异而放大的中心获得时。
Inter-observer variation has been examined for a number of histomorphometric indices in 20 normal human iliac crest biopsies. Quantitation was performed using an eye-piece graticule and eye-piece micrometer. The same sections were examined by two observers and the methodology was identical. Intra-observer variation was also assessed. Significant inter-observer differences were found for the measurement of total trabecular bone volume, osteoid volume and surface, double plus single and double tetracycline labeled surfaces, and the mean osteoid seam width. The percentage variance due to inter-observer variation was highest for osteoid surface and volume, total resorption surface, and mean osteoid seam width. Intra-observer variation in both observers was small. We conclude that a large inter-observer variation may occur in the measurement of a number of histomorphometric indices, even when section preparation and methodology are identical. Caution should be used in basing the diagnosis of metabolic bone disease on strictly defined control data from other observers, particularly when this has been obtained from centers where the effects of inter-observer variation may be magnified by differences in methodology.