α-Glucosidase inhibitory effect by the flower buds of Tussilago farfara L.
α-Glucosidase inhibitory effect by the flower buds of Tussilago farfara L.
复制标题
DOI：
10.1016/j.foodchem.2007.07.064
复制
发表时间：
2008-02-01
期刊：
影响因子：
8.8
通讯作者：
Kawabata, Jun
中科院分区：
文献类型：
--
作者：
Gao, Hong;Huang, Yi-Na;Kawabata, Jun
对50种中药药用部位的乙醇提取物进行了大鼠肠道α-葡萄糖苷酶的筛选实验。以麦芽糖为底物的百合花蕾乙醇提取物对麦芽糖酶的抑制活性最高。提供了酶分析引导的这种提取物的分级。3，4-二咖啡酰奎宁酸(1)、3，5-二咖啡酰奎宁酸(2)、4，5-二咖啡酰奎宁酸(3)和芦丁(4)。化合物1、2和3具有较强的麦芽糖酶抑制活性，其IC50值分别为0.91 mM、0.90 mM和0.89 mM。比较了1-3、绿原酸(5)、奎尼酸(6)和咖啡酸(7)的活性，表明连接在奎宁酸核心上的咖啡酰基的数量对活性很重要。芦丁(4)表现出中等活性，在1 mM的浓度下对麦芽糖酶活性有41%的抑制作用。这是首次报道哺乳动物α-葡萄糖苷酶对法法氏菌的抑制作用，并从该草本植物中分离得到1、2和3个化合物。这些结果表明，法法拉提取物可用于抗糖尿病。(C)2007爱思唯尔有限公司。保留所有权利。
Methanolic extracts from the medicinal parts of 50 traditional Chinese herbs were tested in screening experiments for rat intestinal alpha-glucosidase. The methanolic extract from flower buds of Tussilago farfara L. (Compositae) showed the highest maltase inhibitory activity, with maltose as a substrate. Enzyme assay-guided fractionation of this extract afforded. 3,4-dicaffeoylquinic acid (1), 3,5-dicaffeoyl-quinic acid (2), 4,5-dicaffeoylquinic acid (3) and rutin (4), and the structures of these compounds were elucidated on the basis of MS and NMR data analyses. Compounds 1, 2 and 3 showed comparative maltase inhibitory activities, and the IC50 values were 0.91 mM, 0.90 mM and 0.89 mM, respectively. Comparison of the activities of 1-3, chlorogenic acid (5), quinic acid (6) and caffeic acid (7) suggested that the number of caffeoyl groups attached to a quinic acid core were important for the potency. Rutin (4) showed moderate activity and inhibited 41 % of maltase activity at a concentration of 1 mM. This is the first report on mammalian alpha-glucosidase inhibition of T farfara and the isolation of 1, 2 and 3 from this herb species. These results suggest a use of the extract of T farfara for antidiabetes. (c) 2007 Elsevier Ltd. All rights reserved.