Proliferation of granule cell precursors in the dentate gyrus of adult monkeys is diminished by stress
Proliferation of granule cell precursors in the dentate gyrus of adult monkeys is diminished by stress
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DOI：
10.1073/pnas.95.6.3168
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发表时间：
1998-03-17
影响因子：
11.1
通讯作者：
Fuchs, E
中科院分区：
文献类型：
--
作者：
Gould, E;Tanapat, P;Fuchs, E
尽管成年大鼠和树鼩的齿状回中仍有颗粒细胞的存在，但这种现象在成年灵长类动物的齿状回中尚未得到证实。为了确定成年灵长类动物的齿状回中是否产生神经元，将成年绒猴（Callithrix jacchus）注射BrdU并在2小时或3周后灌注。BrdU是胸苷类似物，其在S期掺入增殖细胞。大量的成年猴的齿状回的细胞纳入BrdU，这些细胞中约80%的颗粒神经元的形态特征，并表示由3周的时间点的神经元标记。以往的研究表明，成年大鼠和树鼩的齿状回颗粒细胞前体细胞的增殖可受到应激的抑制。为了测试一种令人厌恶的经历是否对灵长类动物大脑中的细胞增殖有类似的影响，成年绒猴被暴露在一种居住者-入侵者的压力模型中。在这种条件下1小时后，入侵的猴子注射BrdU，2小时后灌注。将入侵猴子的齿状回中增殖细胞的数量与未受压力的对照猴子进行比较。我们发现，一个单一的暴露于这种压力的经验，导致这些增殖细胞的数量显着减少，我们的研究结果表明，神经元产生在成年猴子的齿状回和前体细胞增殖的速度可以受到压力的经验。
Although granule cells continue to be added to the dentate gyrus of adult rats and tree shrews, this phenomenon has not been demonstrated in the dentate gyrus of adult primates. To determine whether neurons are produced in the dentate gyrus of adult primates, adult marmoset monkeys (Callithrix jacchus) were injected with BrdU and perfused 2 hr or 3 weeks later, BrdU is a thymidine analog that is incorporated into proliferating cells during S phase. A substantial number of cells in the dentate gyrus of adult monkeys incorporated BrdU and approximate to 80% of these cells had morphological characteristics of granule neurons and expressed a neuronal marker by the 3-week time point. Previous studies suggest that the proliferation of granule cell precursors in the adult dentate gyrus can be inhibited by stress in rats and tree shrews. To test whether an aversive experience has a similar effect on cell proliferation in the primate brain, adult marmoset monkeys were exposed to a resident-intruder model of stress. After 1 hr in this condition, the intruder monkeys were injected with BrdU and perfused 2 hr later. The number of proliferating cells in the dentate gyrus of the intruder monkeys was compared with that of unstressed control monkeys. We found that a single exposure to this stressful experience resulted in a significant reduction in the number of these proliferating cells, Our results suggest that neurons are produced in the dentate gyrus of adult monkeys and that the rate of precursor cell proliferation can be affected by a stressful experience.