Antibacterial iodine-supported titanium implants
Antibacterial iodine-supported titanium implants
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DOI：
10.1016/j.actbio.2010.11.036
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发表时间：
2011-04-01
影响因子：
9.7
通讯作者：
Tsuchiya, H.
中科院分区：
文献类型：
--
作者：
Shirai, T.;Shimizu, T.;Tsuchiya, H.
深部感染仍然是骨科种植手术的严重并发症。为了减少种植体相关感染的发生率，人们提出了几种生物材料表面处理方法。本研究旨在评价载碘钛(Ti-I-2)的抗菌活性及其对种植体后感染的影响，并确定其作为生物材料的潜在适宜性。由于易于制作败血症模型，本实验中使用外固定钉作为试验性植入物。用改进的日本工业标准方法测量金属的抗菌活性。通过将植入物暴露于金黄色葡萄球菌或大肠杆菌，并比较病原体对Ti-I-2与不锈钢和钛对照的反应来评估活性。Ti-I-2对细菌定植的抑制作用明显强于对照金属。此外，通过计数金属表面形成的集落数量来评估细胞相容性。三种金属的成纤维细胞集落形成量相同。采用日本大白兔作为活体动物模型。6只兔双侧股骨各插入3枚钢针进行组织学观察。检查针孔部位，并对感染和炎症进行分级。与Ti-I-2钉结合使用时，感染和炎性改变的迹象较少。此外，通过钉的类骨形成表面来评价种植体的骨传导性。钛-I-2和钛钉周围有连续的骨形成，而不锈钢钉周围几乎没有类骨形成。这些发现表明，Ti-I-2具有抗菌活性和细胞相容性。因此，Ti-I-2极大地降低了种植体感染的发生率，并显示出作为生物材料的特殊前景。(C)2010年Acta Materialia Inc.由爱思唯尔有限公司出版。版权所有。
Deep infection remains a serious complication in orthopedic implant surgery. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. This study focused on evaluating the antibacterial activity of iodine-supported titanium (Ti-I-2) and its impact on post-implant infection, as well as determining the potential suitability of Ti-I-2 as a biomaterial. External fixation pins were used in this experiment as trial implants because of the ease of making the septic models. The antibacterial activity of the metal was measured using a modification of the Japanese Industrial Standards method. Activity was evaluated by exposing the implants to Staphylococcus aureus or Escherichia call and comparing reaction of pathogens to Ti-I-2 vs. stainless steel and titanium controls. Ti-I-2 clearly inhibited bacterial colonization more than the control metals. In addition, cytocompatibility was assessed by counting the number of colonies that formed on the metals. The three metals showed the same amount of fibroblast colony formation. Japanese white rabbits were used as an in vivo model. Three pins were inserted into both femora of six rabbits for histological analysis. Pin sites were inspected and graded for infection and inflammation. Fewer signs of infection and inflammatory changes were observed in conjunction with the Ti-I-2 pins. Furthermore, osteoconductivity of the implant was evaluated with osteoid formation surface of the pin. Consecutive bone formation was observed around the Ti-I-2 and titanium pins, while little osteoid formation was found around the stainless steel pins. These findings suggest that Ti-I-2 has antimicrobial activity and exhibits cytocompatibility. Therefore, Ti-I-2 substantially reduces the incidence of implant infection and shows particular promise as a biomaterial. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.