A CDNA CLONE ENCODING A PHOTOSYSTEM-I PROTEIN WITH HOMOLOGY TO PHOTOSYSTEM-II CHLOROPHYLL A/B-BINDING POLYPEPTIDES
A CDNA CLONE ENCODING A PHOTOSYSTEM-I PROTEIN WITH HOMOLOGY TO PHOTOSYSTEM-II CHLOROPHYLL A/B-BINDING POLYPEPTIDES
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DOI：
10.1073/pnas.84.24.8844
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发表时间：
1987-12-01
影响因子：
11.1
通讯作者：
CASHMORE, AR
中科院分区：
文献类型：
--
作者：
HOFFMAN, NE;PICHERSKY, E;CASHMORE, AR
我们在这里报告的分离和核苷酸序列的一个完整的cDNA克隆编码的光系统I（PS I）的多肽，识别的单克隆抗体对光系统II（PS II）叶绿素a/b-结合（CAB）蛋白。推测的PS I蛋白序列与PS II CAB序列的同源性为30%，其中两个长片段与PS II CAB多肽中的相应片段的同源性分别为50%和65%。即使这个PS I CAB蛋白的序列是基本上不同的PS II CAB序列，它们的亲水性图是非常相似的，并建议他们都穿越类囊体膜三次。PS I CAB多肽的一个区段显示出与功能类似物β的相似性。紫色细菌触角蛋白的亚基。与此相反，没有观察到这些细菌蛋白质和PS II CAB多肽之间的同源性。
We report here the isolation and nucleotide sequence of a complete cDNA clone encoding a photosystem I (PS I) polypeptide that is recognized by a monoclonal antibody made against photosystem II (PS II) chlorophyll a/b-binding (CAB) proteins. The deduced sequence of this PS I protein shows 30% overall identity to PS II CAB sequences, and two long segments within this protein show 50% and 65% identity to the corresponding segments in the PS II CAB polypeptides. Even though the sequence of this PS I CAB protein is substantially divergent PS II CAB sequences, their hydropathy plots are very similar and suggest they all traverse the thylakoid membrane three times. A segment of the PS I CAB polypeptide shows simliarity to the functionally analogous .beta. subunits of the antenna proteins of purple bacteria. In contrast, no homology was observed between these bacterial proteins and PS II CAB polypeptides.