Visual pigment phosphorylation but not transducin translocation can contribute to light adaptation in zebrafish cones
Visual pigment phosphorylation but not transducin translocation can contribute to light adaptation in zebrafish cones
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DOI：
10.1016/s0896-6273(04)00086-8
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发表时间：
2004-03-25
期刊：
影响因子：
16.2
通讯作者：
Dunn, FA
中科院分区：
文献类型：
--
作者：
Kennedy, MJ;Dunn, FA
视锥细胞感光器适应光线的能力是非同寻常的。在这项研究中，我们评估了两个生化过程，视觉色素磷酸化和转导蛋白转移，以帮助斑马鱼锥体的光适应能力。由于细胞质中的钙离子调节光适应，因此我们研究了这些过程对光和钙离子的敏感性。胞浆内的钙离子调节光刺激的磷酸化位点。出乎意料的是，我们发现钙离子也调节着未漂白的锥形色素的磷酸化程度。免疫细胞化学分析表明，光和胞浆中的钙离子都不影响转导蛋白在斑马鱼锥体中的定位。
The ability of cone photoreceptors to adapt to light is extraordinary. In this study we evaluated two biochemical processes, visual pigment phosphorylation and transducin translocation, for their ability to contribute to light adaptation in zebrafish cones. Since cytoplasmic Ca2+ regulates light adaptation, the sensitivities of these processes to both light and Ca2+ were examined. Cytoplasmic Ca2+ regulates the sites of light-stimulated phosphorylation. Unexpectedly, we found that Ca2+ also regulates the extent of phosphorylation of unbleached cone pigments. Immunocytochemical analyses revealed that neither light nor cytoplasmic Ca2+ influences the localization of transducin in zebrafish cones.