Transcriptome analysis of wheat inoculated with Fusarium graminearum.
Transcriptome analysis of wheat inoculated with Fusarium graminearum.
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DOI：
10.3389/fpls.2015.00867
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发表时间：
2015
影响因子：
5.6
通讯作者：
Unver T
中科院分区：
文献类型：
--
作者：
Erayman M;Turktas M;Akdogan G;Gurkok T;Inal B;Ishakoglu E;Ilhan E;Unver T
植物经常暴露于引起生物胁迫的真菌、细菌和病毒等微生物。赤霉病 (FHB) 是一种具有经济风险的小麦病害，由禾谷镰刀菌 (Fg) 感染引起。中度敏感（cv.“Mizrak 98”）和敏感（cv.“Gun 91”）冬季型面包小麦品种在暴露于 Fg 感染后进行转录分析。为了检查小麦对病原体的早期反应，我们使用 12X135K 微阵列芯片测量了接种后 12 小时 (hai) 的中度敏感 (MS) 和敏感品种的模拟根冠和病原体接种根冠的基因表达变化。转录组分析显示，在 39,179 个转录本中，微阵列中的 3668 个基因至少在一次比较中受到显着调节。大多数差异调节转录本与疾病反应和基因表达机制相关。当对品种进行比较时，品种内的许多转录本和表达变化有所不同。特别是膜相关转录本被检测为差异表达。此外，不同的转录因子在品种之间表现出显着的倍数变化值。这项研究为了解所选品种对 12 公顷 Fg 的早期反应提供了新的见解。通过 KEGG 分析，我们观察到变化最大的转录本与淀粉和蔗糖代谢以及糖异生途径相关。
Plants are frequently exposed to microorganisms like fungi, bacteria, and viruses that cause biotic stresses. Fusarium head blight (FHB) is an economically risky wheat disease, which occurs upon Fusarium graminearum (Fg) infection. Moderately susceptible (cv. “Mizrak 98”) and susceptible (cv. “Gun 91”) winter type bread wheat cultivars were subjected to transcriptional profiling after exposure to Fg infection. To examine the early response to the pathogen in wheat, we measured gene expression alterations in mock and pathogen inoculated root crown of moderately susceptible (MS) and susceptible cultivars at 12 hours after inoculation (hai) using 12X135K microarray chip. The transcriptome analyses revealed that out of 39,179 transcripts, 3668 genes in microarray were significantly regulated at least in one time comparison. The majority of differentially regulated transcripts were associated with disease response and the gene expression mechanism. When the cultivars were compared, a number of transcripts and expression alterations varied within the cultivars. Especially membrane related transcripts were detected as differentially expressed. Moreover, diverse transcription factors showed significant fold change values among the cultivars. This study presented new insights to understand the early response of selected cultivars to the Fg at 12 hai. Through the KEGG analysis, we observed that the most altered transcripts were associated with starch and sucrose metabolism and gluconeogenesis pathways.