FLUORESCENCE MICROSCOPY OF SINGLE MOLECULES
FLUORESCENCE MICROSCOPY OF SINGLE MOLECULES
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DOI：
10.1016/0009-2614(94)87003-9
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发表时间：
1994-01-21
影响因子：
2.8
通讯作者：
WILD, UP
中科院分区：
文献类型：
--
作者：
GUTTLER, F;IRNGARTINGER, T;WILD, UP
在1.8K的温度下，用荧光激发光谱法在显微镜下对并五苯单分子进行了研究。该显微镜具有3 μ m的分辨率，并允许一个单一的发射分子的位置与0.5 μ m的精度，在235 μ m-175 μ m的总视场。通过以2 MHz的步长增加592.344nm处的光的激发频率，已经记录了133个图像。每个单分子具有宽度约为14 MHz的洛伦兹吸收曲线，可以在10个连续图像中观察到。单分子的“位置”由空间域中的x和y坐标以及光谱域中的吸收频率表征。
Using fluorescence excitation spectroscopy single pentacene molecules were studied under a microscope at a temperature of 1.8 K. The microscope has a resolution of 3 mu m and allows the location of a single emitting molecule with an accuracy of 0.5 pm, in a total viewing field of 235 mu m 175 mu m. 133 images have been recorded by increasing the excitation frequency of the light at 592.344 nm in steps of 2 MHz. Each single molecule has a Lorentzian absorption profile with a width of about 14 MHz and can be observed in 10 consecutive images. The ''position'' of the single molecule is characterized by the x- and y-coordinates in the spatial domain and by its absorption frequency in the spectral domain.