Tumor Margin Detection Using Quantitative NIRF Molecular Imaging Targeting EpCAM Validated by Far Red Gene Reporter iRFP
Tumor Margin Detection Using Quantitative NIRF Molecular Imaging Targeting EpCAM Validated by Far Red Gene Reporter iRFP
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DOI：
10.1007/s11307-013-0637-8
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发表时间：
2013-10-01
影响因子：
3.1
通讯作者：
Sevick-Muraca, Eva M.
中科院分区：
文献类型：
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作者：
Zhu, Banghe;Wu, Grace;Sevick-Muraca, Eva M.
宽视场手术切除减少了残留疾病的机会，但当切除接近关键结构时，也可能导致毁容和毁灭性的发病率。我们假设近红外荧光（NIRF）成像可以准确检测肿瘤边缘，用于图像引导切除。采用nif标记的抗上皮细胞粘附分子（EpCAM）抗体，建立人前列腺癌（PCa）原位模型，评估原发肿瘤边缘。PCa细胞稳定表达远红色荧光基因报告基因iRFP，可以与NIRF信号共定位，直接评估肿瘤边缘。利用受体工作特性分析，远红色荧光与原发和转移性病变的标准病理相对照，准确率为> 96%。与使用NIRF标记的同型对照抗体相比，使用epcam靶向抗体进行定量NIRF成像可以更准确地检测原发肿瘤边缘。NIRF分子成像可以实时准确地评估肿瘤边缘。
Wide-field surgical excision reduces the chance of residual disease, but can also lead to disfigurement and devastating morbidities when resection is close to critical structures. We hypothesize that near-infrared fluorescence (NIRF) imaging can enable accurate detection of tumor margins for image-guided resection.An orthotopic model of human prostate cancer (PCa) was used to assess primary tumor margins using a NIRF-labeled antibody against epithelial cell adhesion molecule (EpCAM). PCa cells stably expressing far red fluorescent gene reporter, iRFP, enabled colocalization with NIRF signals for direct assessment of tumor margins.Using receiver operating characteristic analysis, far red fluorescence was validated against standard pathology of primary and metastatic lesions with > 96 % accuracy. Primary tumor margins were more accurately detected by quantitative NIRF imaging using the EpCAM-targeting antibody as compared to a NIRF-labeled isotype control antibody.NIRF molecular imaging may enable real-time and accurate assessment of tumor margins.