An accumulation of two populations of dendritic cells in skin-draining lymph nodes in response to the expression of thymic stromal lymphopoietin in the skin
An accumulation of two populations of dendritic cells in skin-draining lymph nodes in response to the expression of thymic stromal lymphopoietin in the skin
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DOI：
10.1016/j.cellimm.2020.104116
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发表时间：
2020-07-01
影响因子：
4.3
通讯作者：
Yagi, Junji
中科院分区：
文献类型：
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作者：
Omori-Miyake, Miyuki;Watarai, Hiroshi;Yagi, Junji
胸腺基质淋巴细胞生成素（TSLP）作用于树突状细胞（DC），其使辅助性T（Th）细胞成为2型细胞因子产生细胞。最近，已经发现了一组不同的TSLP应答性DC群体。在这里，我们鉴定了两个群体的CD 103（Io）EpCAM（hi）迁移性DC（部分I和部分II），其响应于小鼠皮肤中表达的TSLP而在皮肤引流淋巴结中积累。具有CD 11b（+）PDL 2（hi）表达的级分I DC引发幼稚Th细胞分化为分泌IFN-γ、IL-17 A和IL-22的细胞，而具有CD 11b（Io）PDL 2（hi）表达的级分II DC引发幼稚Th细胞分化为分泌IL-4、IL-5、IL-9、IL-13和IL-10的细胞。部分I DC通过IL-4 R α信号传导途径从皮肤迁移，而部分II DC部分通过TSLPR信号传导途径迁移。所有这些都表明，至少两种具有不同功能和途径的CD 103（Io）EpCAM（hi）DC群体可以响应皮肤中的TSLP表达而迁移。
Thymic stromal lymphopoietin (TSLP) acts on dendritic cells (DCs), which prime helper T (Th) cells to become type 2 cytokine producing cells. Recently, a different set of populations of TSLP-responsive DCs has been discovered. Here, we identified two populations of CD103(Io)EpCAM(hi) migratory DCs (fraction I and fraction II) that accumulated in skin-draining lymph nodes in response to TSLP expressed in the mouse skin. Fraction I DCs with CD11b(+)PDL2(hi) expression primed naive Th cells to differentiate into cells secreting IFN-gamma, IL-17A and IL-22, while fraction II DCs with CD11b(Io)PDL2(hi) expression primed naive Th cells to differentiate into cells secreting IL-4, IL-5, IL-9, IL-13 and IL-10. Fraction I DCs migrated from the skin via IL-4R alpha signaling pathway, whereas fraction II DCs migrated partially via TSLPR signaling pathway. All suggest that at least two populations of CD103(Io)EpCAM(hi) DCs with distinct functions and pathways could migrate in response to TSLP expression in the skin.