IMMORTALIZATION OF MOUSE INTESTINAL EPITHELIAL-CELLS BY THE SV40-LARGE T-GENE - PHENOTYPIC AND IMMUNE CHARACTERIZATION OF THE MODE-K CELL LINE
IMMORTALIZATION OF MOUSE INTESTINAL EPITHELIAL-CELLS BY THE SV40-LARGE T-GENE - PHENOTYPIC AND IMMUNE CHARACTERIZATION OF THE MODE-K CELL LINE
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DOI：
10.1016/0022-1759(93)90329-6
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发表时间：
1993-11-05
影响因子：
2.2
通讯作者：
KAISERLIAN, D
中科院分区：
文献类型：
--
作者：
VIDAL, K;GROSJEAN, I;KAISERLIAN, D
通过鼠嗜亲性病毒将SV 40大T基因转移至小鼠小肠，使肠上皮细胞永生化。所得细胞系表达SV 40大T mRNA，并表现出正常肠上皮细胞的形态学和表型特征，包括细胞间连接，以及细胞角蛋白、绒毛蛋白、多聚Ig受体（即，分泌成分）和血管活性肠肽受体。所有表达细胞表面主要组织相容性复合物I类分子，但细胞表面II类抗原检测不到。使用从小鼠十二指肠建立的MODE-K细胞系进行抗原呈递的功能研究。MODE-K细胞的干扰素-γ处理导致高水平的II类分子表达，以及通过功能性II类分子加工天然蛋白抗原并将其呈递给特异性CD 4 + T细胞杂交瘤的能力。这些数据表明，MODE-K细胞系是一个合适的模型，用于分析肠上皮细胞在粘膜免疫中的功能。
Intestinal epithelial cells from the mouse small intestine were immortalized by SV40 large T gene transfer through a murine ecotropic virus. The resulting cell lines expressed the SV40 large T mRNA and exhibited morphological and phenotypic characteristics of normal enterocytes, including intercellular junctions, and expression of cytokeratin, villin, poly-Ig receptor (i.e., secretory component) and vasoactive intestinal peptide receptors. All expressed cell surface major histocompatibility complex class I molecules, but cell surface class II antigens were undetectable. Functional studies on antigen presentation were carried out using the MODE-K cell line established from the mouse duodenum. Interferon-gamma treatment of MODE-K cells resulted in a high level of class II molecule expression, and the ability to process and present native protein antigens to specific CD4+ T-cell hybridomas, via functional class II molecules. These data suggest that the MODE-K cell line is a suitable model for the analysis of intestinal epithelial cell function in mucosal immunity.