Suppression of UVA-mediated release of labile iron by epicatechin - A link to lysosomal protection
Suppression of UVA-mediated release of labile iron by epicatechin - A link to lysosomal protection
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DOI：
10.1016/j.freeradbiomed.2006.06.008
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发表时间：
2006-10-15
影响因子：
7.4
通讯作者：
Tyrrell, Rex M.
中科院分区：
文献类型：
--
作者：
Basu-Modak, Sharmila;Ali, Dalia;Tyrrell, Rex M.
UVA（320-380 nm）辐射在细胞中产生氧化应激，并导致人体皮肤细胞中潜在破坏性不稳定铁池的立即释放。将培养的皮肤成纤维细胞用生理相关浓度的表儿茶素（EC）（存在于食物中的类黄酮植物成分）或甲基化表儿茶素（3 '-O-甲基表儿茶素，MeOEC）（其主要的人体代谢物）处理数小时，防止这种铁释放。EC和MeOEC有效性的相似性反对螯合作用作为铁去除的机制。基于溶酶体完整性测量的证据强烈支持儿茶素保护溶酶体免受UVA破坏的假设。这种损伤通常会导致蛋白酶的释放，而蛋白酶的释放先前已被证明会导致铁蛋白降解和不稳定铁的释放。(c)2006年爱思唯尔公司All rights reserved.
UVA (320-380 nm) radiation generates an oxidative stress in cells and leads to an immediate release of potentially damaging labile iron pools in human skin cells. Treatment of cultured skin fibroblasts for several hours with physiologically relevant concentrations of either epicatechin (EC), a flavonoid plant constituent present in foods, or methylated epicatechin (3'-O-methyl epicatechin, MeOEC), its major human metabolite, prevents this iron release. The similarity of the effectiveness of EC and MeOEC argues against chelation as the mechanism of iron removal. Evidence based on measurements of lysosomal integrity strongly supports the hypothesis that the catechins protect against lysosomal destruction by UVA. Such damage would normally lead to protease release, which has been previously shown to cause ferritin degradation and release of labile iron. (c) 2006 Elsevier Inc. All rights reserved.