Preparation of Extracellular Matrices Produced by Cultured Corneal Endothelial and PF‐HR9 Endodermal Cells
Preparation of Extracellular Matrices Produced by Cultured Corneal Endothelial and PF‐HR9 Endodermal Cells
复制标题
DOI：
10.1002/0471143030.cb1004s01
复制
发表时间：
1999-01
影响因子：
--
通讯作者：
I. Vlodavsky
中科院分区：
文献类型：
--
作者：
I. Vlodavsky
ECM是一种有组织的胶原、蛋白聚糖和糖蛋白复合物，它们相互作用产生高度稳定的结构，使细胞在体内迁移、增殖、分化和生存。培养的牛角膜内皮细胞（BCE）和PF - HR9内胚层细胞产生的内皮细胞与塑料紧密结合，在体内的形态、分子组成和生物活性上与内皮下和上皮下基底膜非常相似。本单元介绍了这些ecm的制备方法，它们的性质和它们的细胞效应。
The ECM is an organized complex of collagens, proteoglycans, and glycoproteins, all interacting to produce a highly stable structure upon which cells migrate, proliferate, differentiate, and survive in vivo. Cultured bovine corneal endothelial (BCE) cells and PF‐HR9 endodermal cells produce underlying ECMs that adhere strongly to plastic and closely resemble subendothelial and subepithelial basement membranes in vivo in their morphology, molecular composition, and biological activities. This unit describes the methods for preparation of these ECMs, their properties, and their cellular effects.