A reproduceable in situ xenograft model of spinal glioma
A reproduceable in situ xenograft model of spinal glioma
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可复制的脊髓胶质瘤原位异种移植模型
DOI：
10.1016/j.jneumeth.2020.108928
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发表时间：
2020-09
影响因子：
3
通讯作者：
Yuntao Lu
中科院分区：
文献类型：
--
作者：
Zhijian Weng;Liyi Ma;Junjie Li;Qiang Zhou;Lin Peng;Hong Li;Lei Chen;Zong Xin;Linyong Shi;Songtao Qi;Yuntao Lu
脊髓胶质瘤是一种易复发的神经系统肿瘤，目前尚无特异性的预后分子标志物。因此，迫切需要一种稳定、可重复的脊髓胶质瘤动物模型。新方法我们建立了一种新的裸鼠脊髓胶质瘤原位移植瘤模型。本研究将肿瘤植入裸鼠颈髓内，模拟原肿瘤的病理特征。结果通过解剖学实验，我们发现小鼠颈椎板较薄，椎间隙较宽，粘连肌肉较易分离。根据脊髓切片检查，我们确定的最佳穿刺点位于肩胛骨中点之间的水平上的后正中线外侧0.9mm，深度为0.9mm。在裸鼠异种移植瘤实验中，植入的肿瘤组织保留了原肿瘤的病理学特征。与现有方法的比较该模型采用颈髓作为穿刺部位，采用患者源性原发肿瘤细胞，这在以往从未进行过。肿瘤细胞可以直接注射而不损伤椎板。结论本研究建立了一种稳定可靠的脊髓胶质瘤动物模型，为进一步的分子生物学研究和靶向治疗奠定了基础。
BackgroundSpinal glioma is a nervous system tumor that tends to relapse and has no specific prognostic molecular biomarkers. Thus, a stable and reproduceable animal research model of spinal glioma is urgently needed.New methodWe established a new in situ tumor xenograft model of spinal glioma using nude mice. In this study, we implanted tumors into the cervical spinal cord of nude mice to mimic the pathological characteristics of the original tumors.ResultsThrough anatomical experiments, we found that the cervical lamina of mice was thinner, the intervertebral space was much wider, and the adhesion muscles were more easily separated. According to the examination of spinal cord sections, the best puncture point we identified was located 0.9 mm lateral to the posterior median line at the level of the line between the midpoints of the scapulae and at a depth of 0.9 mm. In the nude mouse xenograft experiment, the implanted tumor tissue retained the pathological characteristics of the original tumor.Comparison with existing method(s)This model used the cervical spinal cord as the puncture site and patient-derived primary tumor cells, which has never been performed before. Tumor cells could be injected directly without damaging the lamina. Thus, we could reduce the risk of man-made spinal cord injury and infection and avoid destroying the stability and integrity of the spine.ConclusionsThis study established a stable and reliable animal model of spinal glioma for further molecular research and targeted therapy development.
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