Analysis of gene expression with cDNA microarrays in rat brain after 7 and 42 days of oral lithium administration
Analysis of gene expression with cDNA microarrays in rat brain after 7 and 42 days of oral lithium administration
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DOI：
10.1016/s0361-9230(01)00744-4
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发表时间：
2002-01-15
影响因子：
3.8
通讯作者：
Manickam, P
中科院分区：
文献类型：
--
作者：
Bosetti, F;Seemann, R;Manickam, P
采用微阵列技术检测氯化锂喂养7天（亚急性）或42天（慢性）大鼠脑内基因表达谱。在第7天和第42天，脑锂浓度分别为0.39 mM和0.79 mM（治疗相关）。在微阵列中的4132个基因中，有25个基因在治疗7天后下调了至少两倍，没有一个基因上调。在42天，50个基因的表达下调至少2倍，没有任何上调。锂处理7天对42天下调的50个基因中37个的表达没有显著影响。在42天改变表达的基因编码了许多受体、蛋白激酶、转录和翻译因子、能量代谢标志物和信号转导。因此，治疗相关浓度的慢性锂降低了参与多种信号传导和其他途径的大量基因的表达，而没有在相当程度上增加表达。(C) 2002 Elsevier Science。
The gene expression profile in rat brain was examined using microarrays in rats fed lithium chloride for 7 days (subacute) or 42 days (chronic). Brain lithium concentrations were 0.39 mM and 0.79 mM (therapeutically relevant), at 7 and 42 days, respectively. Of the 4132 genes represented in the microarrays, 25 genes were downregulated by at least twofold and none was upregulated after 7 days of treatment. Expression of 50 genes was downregulated by at least two-fold at 42 days, without any being upregulated. Lithium treatment for 7 days did not affect at a measurable extent expression of 37 of the 50 genes that were downregulated at 42 days. Genes whose expression was changed at 42 days coded for a number of receptors, protein kinases, transcription and translation factors, markers of energy metabolism, and signal transduction. Thus, chronic lithium at a therapeutically relevant concentration reduced expression of a large number of genes involved in multiple signaling and other pathways, without increasing expression at a comparable extent. (C) 2002 Elsevier Science.