Effect of subinhibitory antibiotic concentrations on polysaccharide intercellular adhesin expression in biofilm-forming Staphylococcus epidermidis
Effect of subinhibitory antibiotic concentrations on polysaccharide intercellular adhesin expression in biofilm-forming Staphylococcus epidermidis
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DOI：
10.1128/aac.44.12.3357-3363.2000
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发表时间：
2000-12-01
影响因子：
4.9
通讯作者：
Ziebuhr, W
中科院分区：
文献类型：
--
作者：
Rachid, S;Ohlsen, K;Ziebuhr, W
生物膜的产生是表皮葡萄球菌聚合物相关感染发病机制的重要步骤，依赖于icaADBC操纵子的表达，导致多糖细胞间粘附素的合成。构建了大肠杆菌中ica启动子与β -半乳糖苷酶基因lacZ的染色体编码融合报告基因，研究了环境因素和不同抗生素亚抑制浓度对表皮葡萄球菌中ica表达的影响。结果表明，S，表皮生物膜的形成是由外界胁迫（即高温和渗透压）诱导的。亚抑制浓度的四环素和半合成链状gramin抗生素quinupritin - dalopristin可使ica表达提高9- 2倍，而青霉素、oxacillin、氯霉素、克林霉素、庆大霉素、氧氟沙星、万古霉素和替柯普兰对ica表达无影响。在远低于红霉素抑制浓度的情况下，观察到ica表达的微弱（即2.5倍）诱导。结果通过ica转录的Northern blot分析和生物膜形成的比色定量分析得到证实。
Biofilm production is an important step in the pathogenesis of Staphylococcus epidermidis polymer-associated infections and depends on the expression of the icaADBC operon leading to the synthesis of a polysaccharide intercellular adhesin. A chromosomally encoded reporter gene fusion between the ica promoter and the beta-galactosidase gene lacZ from Escherichia coli was constructed and used to investigate the influence of both environmental factors and subinhibitory concentrations of different antibiotics on ica expression in S. epidermidis. It was shown that S, epidermidis biofilm formation is induced by external stress (i.e., high temperature and osmolarity). Subinhibitory concentrations of tetracycline and the semisynthetic streptogramin antibiotic quinupristin-dalfopristin were found to enhance ica expression 9- to Ii-fold, whereas penicillin, oxacillin, chloramphenicol, clindamycin, gentamicin, ofloxacin, vancomycin, and teicoplanin had no effect on ica expression. A weak (i.e., 2.5-fold) induction of ica expression was observed far subinhibitory concentrations of erythromycin. The results were confirmed by Northern blot analyses of ica transcription and quantitative analyses of biofilm formation in a colorimetric assay.