Over‐transcription of genes in a parathion‐resistant strain of mosquito Culex pipiens quinquefasciatus
Over‐transcription of genes in a parathion‐resistant strain of mosquito Culex pipiens quinquefasciatus
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DOI：
10.1111/1744-7917.12106
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发表时间：
2015-02
期刊：
影响因子：
4
通讯作者：
Wei Wang;Siwei Liu;Yangyang Liu;C. Qiao;Shaoliang Chen;F. Cui
中科院分区：
文献类型：
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作者：
Wei Wang;Siwei Liu;Yangyang Liu;C. Qiao;Shaoliang Chen;F. Cui
杀虫剂抗性是一种进化适应，由于化学杀虫剂的选择压力大、世代快和种群规模大，在蚊子中发展得相当快。识别与杀虫剂抗性相关的基因是了解导致抗性的复杂过程的基础。本研究采用抑制性消减杂交和互补DNA基因芯片技术相结合的方法，比较了致倦库蚊对硫磷抗性和易感基因的转录水平。从抗性蚊虫消减文库中筛选出278个菌落，其中38个菌落的免疫印迹信号在抗性品系中比敏感品系强2倍以上。测序结果表明，38个菌落可与致倦库蚊的12个基因相匹配。在抗性品系中，有8个基因被证实过表达两倍以上。这些基因编码糜蛋白酶-1、谷胱甘肽S转移酶、脂肪酶3、幼虫血清蛋白1β链、细胞色素b、线粒体核糖体大亚基、28S rRNA以及一种功能未知的蛋白质。这项研究是对该蚊种中与有机磷抗性相关的新基因的初步尝试，并为深入了解杀虫剂抗性这一复杂的生理现象提供了依据。
Insecticide resistance is an evolutionary adaptation that develops quite quickly in mosquitoes because of the high selection pressure of chemical insecticides, rapid generation time and large population size. Identification of genes associated with insecticide resistance is fundamental to understand the complex processes responsible for resistance. We compared the gene transcriptional profiles of parathion‐resistant and ‐susceptible Culex pipiens quinquefasciatus using a combination of suppression subtractive hybridization and complementary DNA (cDNA) microarray techniques. A total of 278 colonies were selected from the resistant‐susceptible mosquito subtractive library, 38 of which showed more than two fold stronger immunoblotting signals in the resistant strain than in the susceptible strain using cDNA microarray selection. The sequencing results showed that the 38 colonies can be matched to 12 genes of C. p. quinquefasciatus. Eight genes were confirmed to be overexpressed by more than two fold in the resistant strain. These genes encode chymotrypsin‐1, theta glutathione S‐transferase, lipase 3, larval serum protein 1 β chain, cytochrome b, mitochondrial ribosomal large subunit, 28S rRNA, and a protein with unknown function. This study serves as a preliminary attempt to identify new genes associated with organophosphate resistance in this mosquito species and provides insights into the complicated physiological phenomenon of insecticide resistance.