Evolutionary adaptation after crippling cell polarization follows reproducible trajectories
Evolutionary adaptation after crippling cell polarization follows reproducible trajectories
复制标题
DOI：
10.7554/elife.09638
复制
发表时间：
2015-10-01
期刊：
影响因子：
7.7
通讯作者：
Murray, Andrew W.
中科院分区：
文献类型：
--
作者：
Laan, Liedewij;Koschwanez, John H.;Murray, Andrew W.
单元由功能模块组织，这些功能模块通常包含组件，这些组件的移除严重损害了模块的功能。尽管它们很重要，但这些成分在生命之树的各个部分之间并不是绝对保守的，这表明细胞可以进化为用不同的蛋白质执行相同的生物学功能。我们在没有重要极性基因BEM1的情况下进化了酿酒酵母1000代。最初，bem 1 Delta谱系的适应性迅速增加，然后在进化结束时慢慢达到其BEM1祖先的90%以上的适应性。对它们的基因组进行测序和监测极化揭示了一个共同的进化轨迹，具有固定的适应性突变序列，每个突变都通过使蛋白质失活来改善细胞极化。我们的研究结果表明，生物体可以在进化上强大的生理破坏性扰动，并表明基因失活的恢复可能会导致细胞生物学重要功能的部件列表中的快速分歧。
Cells are organized by functional modules, which typically contain components whose removal severely compromises the module's function. Despite their importance, these components are not absolutely conserved between parts of the tree of life, suggesting that cells can evolve to perform the same biological functions with different proteins. We evolved Saccharomyces cerevisiae for 1000 generations without the important polarity gene BEM1. Initially the bem1 Delta lineages rapidly increase in fitness and then slowly reach >90% of the fitness of their BEM1 ancestors at the end of the evolution. Sequencing their genomes and monitoring polarization reveals a common evolutionary trajectory, with a fixed sequence of adaptive mutations, each improving cell polarization by inactivating proteins. Our results show that organisms can be evolutionarily robust to physiologically destructive perturbations and suggest that recovery by gene inactivation can lead to rapid divergence in the parts list for cell biologically important functions.