Identification of erythroid-enriched gene expression in the mouse embryonic yolk sac using microdissected cells.
Identification of erythroid-enriched gene expression in the mouse embryonic yolk sac using microdissected cells.
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使用显微解剖细胞鉴定小鼠胚胎卵黄囊中富含红系的基因表达。
DOI：
10.1002/dvdy.21426
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Lloyd,JoyceA
中科院分区：
文献类型：
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作者：
Redmond,LatashaC;Dumur,CatherineI;Archer,KellieJ;Haar,JackL;Lloyd,JoyceA
对控制胚胎红细胞程序的基因知之甚少。使用激光捕获显微切割从胚胎第9.5天卵黄囊的冷冻切片中分离原始红细胞前体细胞和上皮细胞。将RNA样品扩增并标记用于与Affyssin GeneChip小鼠基因组430 A 2.0阵列杂交。91个基因在红系细胞中的表达显著高于上皮细胞。免疫途径分析表明，这些富含红细胞的基因中有许多聚集在高度重要的生物网络中。其中一个网络包含RBTN 2/LMO 2、SCL/TAL 1和EKLF/KLF 1，这是原始红细胞生成所需的极少数基因中的三个。采用定量真实的实时聚合酶链反应验证血小板因子4、reelin、血小板反应蛋白-1和肌盲样1 mRNA是红细胞富集的。这些基因在其他系统中的发育或分化中具有既定的作用，因此是调节原始红细胞生成的良好候选者。这些结果提供了原始红细胞生成过程中表达的基因目录。发展动力学237：436-446，2008年。© 2008 Wiley利斯公司
Little is known about the genes that control the embryonic erythroid program. Laser capture microdissection was used to isolate primitive erythroid precursors and epithelial cells from frozen sections of the embryonic day 9.5 yolk sac. The RNA samples were amplified and labeled for hybridization to Affymetrix GeneChip Mouse Genome 430A 2.0 arrays. Ninety‐one genes are expressed significantly higher in erythroid than in epithelial cells. Ingenuity pathway analysis indicates that many of these erythroid‐enriched genes cluster in highly significant biological networks. One of these networks containsRBTN2/LMO2,SCL/TAL1, andEKLF/KLF1, three of the very few genes required for primitive erythropoiesis. Quantitative real‐time polymerase chain reaction was used to verify thatplatelet factor 4,reelin,thrombospondin‐1, andmuscleblind‐like 1mRNA is erythroid‐enriched. These genes have established roles in development or differentiation in other systems, and are, therefore, good candidates for regulating primitive erythropoiesis. These results provide a catalog of genes expressed during primitive erythropoiesis. Developmental Dynamics 237:436–446, 2008. © 2008 Wiley‐Liss, Inc.