Identification of Group A Streptococcus-Containing Autophagosome-Like Vacuoles
Identification of Group A Streptococcus-Containing Autophagosome-Like Vacuoles
复制标题
含有 A 组链球菌的自噬体样液泡的鉴定
DOI：
10.1007/978-1-0716-0467-0_16
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Nakagawa Ichiro
中科院分区：
文献类型：
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作者：
Nozawa Takashi;Nakagawa Ichiro
A组链球菌（GAS）是人类主要的病原体之一，可侵入非吞噬细胞。通过内吞作用内化的GAS分泌成孔毒素链球菌溶血素O（SLO）逃逸到细胞质中。细胞溶质GAS被自噬膜（含GAS的自噬小体样空泡，GcAV）选择性地捕获并递送至溶酶体进行降解。大自噬（以下简称自噬）是一种高度保守的溶酶体介导的分解代谢过程，对细胞内稳态至关重要。自噬也充当细胞内免疫系统。在本节中，我们描述了如何使用荧光显微镜鉴定感染细胞中的GcAV。
Group A Streptococcus (GAS) is one of the major human pathogens that can invade nonphagocytic cells. GAS internalized through endocytosis secretes the pore-forming toxin Streptolysin O (SLO) to escape into the cytoplasm. The cytosolic GAS is selectively captured by autophagic membranes (GAS-containing autophagosome-like vacuoles, GcAVs) and delivered to lysosomes for degradation. Macroautophagy (referred to as autophagy hereafter) is a highly conserved lysosome-mediated catabolic process, which is critical for cellular homeostasis. Autophagy also acts as an intracellular immune system. In this section, we describe how to identify GcAVs in infected cells using fluorescent microscopy.