Corrigendum to “Aquaporin-11 containing a divergent NPA motif has normal water channel activity” [Biochim. Biophys. Acta 1768 (2007) 688–693]
Corrigendum to “Aquaporin-11 containing a divergent NPA motif has normal water channel activity” [Biochim. Biophys. Acta 1768 (2007) 688–693]
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DOI：
10.1016/j.bbamem.2008.03.001
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发表时间：
2008-06
期刊：
影响因子：
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通讯作者：
Kaya Yakata;Y. Hiroaki;K. Ishibashi;E. Sohara;S. Sasaki;K. Mitsuoka;Y. Fujiyoshi
中科院分区：
文献类型：
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作者：
Kaya Yakata;Y. Hiroaki;K. Ishibashi;E. Sohara;S. Sasaki;K. Mitsuoka;Y. Fujiyoshi
最近，两种新型哺乳动物水通道蛋白 (AQP) AQP 11 和 12 已被鉴定并归类为新 AQP 亚家族（“亚细胞 AQP”）的成员。在该亚家族的成员中，两个天冬酰胺-脯氨酸-丙氨酸（NPA）基序之一在选择性水传导中发挥着至关重要的作用，但并不完全保守。小鼠 AQP11 (mAQP11) 在 Sf9 细胞中表达，并使用去污剂 Fos-choline 10 进行纯化。该蛋白质被重构为脂质体，用于通过停流装置进行水传导研究。 AQP11的单一水渗透率（pf）为1.72±0.03×10−13cm3/s，表明具有不完全保守的NPA基序的亚家族的其他成员也可能具有水通道的功能。
Recently, two novel mammalian aquaporins (AQPs), AQPs 11 and 12, have been identified and classified as members of a new AQP subfamily, the “subcellular AQPs”. In members of this subfamily one of the two asparagine–proline–alanine (NPA) motifs, which play a crucial role in selective water conduction, are not completely conserved. Mouse AQP11 (mAQP11) was expressed in Sf9 cells and purified using the detergent Fos-choline 10. The protein was reconstituted into liposomes, which were used for water conduction studies with a stopped-flow device. Single water permeability (pf) of AQP11 was measured to be 1.72±0.03×10−13cm3/s, suggesting that other members of the subfamily with incompletely conserved NPA motifs may also function as water channels.