Alpha‐smooth‐muscle actin and desmin expressions in human neuroblastoma cell lines
Alpha‐smooth‐muscle actin and desmin expressions in human neuroblastoma cell lines
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DOI：
10.1002/ijc.2910480221
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发表时间：
1991-05
影响因子：
6.4
通讯作者：
T. Sugimoto;H. Ueyama;H. Hosoi;J. Inazawa;Taiji Kato;J. Kemshead;C. Reynolds;A. Gown;H. Mine;T. Sawada
中科院分区：
文献类型：
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作者：
T. Sugimoto;H. Ueyama;H. Hosoi;J. Inazawa;Taiji Kato;J. Kemshead;C. Reynolds;A. Gown;H. Mine;T. Sawada
神经嵴产生多种组织，包括外周神经元、雪旺细胞、黑素细胞和外胚间充质细胞，其中包括大动脉的平滑肌细胞。来源于神经母细胞瘤（一种神经嵴肿瘤）的细胞系表现出至少2种不同的形态学细胞类型，神经母细胞表型（N型）和具有基质细胞特征的上皮样表型（S型）。我们分析了17个人神经母细胞瘤细胞系使用一组单克隆抗体（MAbs）对细胞骨架蛋白。三种神经母细胞瘤细胞系（KP-N-SI、KP-N-YN和SMS-KCN）结合α-平滑肌肌动蛋白抗体。此外，通过间接免疫荧光测定，其中一个细胞系（KP-N-SI）结合抗结蛋白MAb。从上述亲本细胞系获得总共8个克隆化细胞系。这些细胞由N型或5型细胞组成，并通过染色体分析证实与每个亲本细胞系具有相同的神经母细胞瘤来源。通过间接免疫荧光以及二维Western印迹分析，在5型克隆细胞中证实了α平滑肌肌动蛋白和结蛋白。使用人α-平滑肌肌动蛋白mRNA的特异性探针（pSHαSMA-3′UT）通过北方印迹分析证实了这些结果。这些确定了神经母细胞瘤细胞系中α平滑肌肌动蛋白和结蛋白的存在。这些数据表明，除了产生具有神经、许旺细胞和黑素细胞标志物的细胞外，神经母细胞瘤还可以产生表达平滑肌细胞标志物的细胞。
The neural crest gives rise to a variety of tissues, including peripheral neurons, Schwann cells, melanocytes and ectomes‐enchymal cells, which include the smooth‐muscle cells of large arteries. Cell lines derived from neuroblastoma (a neural‐crest tumor) exhibit at least 2 distinct morphological cell types, a neuroblastic phenotype (N‐type) and an epithelial‐like phenotype (S‐type) with characteristics of substrate‐adhesiveness. We have analyzed 17 human neuroblastoma cell lines using a panel of monoclonal antibodies (MAbs) against cytoskeletal proteins. Three neuroblastoma cell lines (KP‐N‐SI, KP‐N‐YN and SMS‐KCN) bound an α‐smooth‐muscle actin antibody. In addition, one of these lines (KP‐N‐SI) bound anti‐desmin MAbs as determined by indirect immunofluorescence. A total of 8 cioned cell lines were obtained from the above parent cell lines. These were composed of either N‐or 5‐type cells and were confirmed to have the same neuroblastoma origin as each parent cell line by chromosomal analysis. Alpha‐smooth‐muscle actin and desmin were demonstrated In the 5‐type cloned cells by indirect immunofluorescence, as well as by 2‐dimensional Western blot analysis. These results were confirmed by Northern blot analysis using a specific probe (pSHαSMA‐3′UT) to human α‐smooth‐muscle actin mRNA. These ascertain the presence of α‐smooth‐muscle actin and desmin in neuroblastoma cell lines. These data show that, in addition to giving rise to cells with neural, Schwann‐cell and melanocyte markers, neuroblastoma can also give rise to the cells expressing smooth‐muscle cell markers.