Dynamic actin filaments are required for stable long-term potentiation (LTP) in area CA1 of the hippocampus
Dynamic actin filaments are required for stable long-term potentiation (LTP) in area CA1 of the hippocampus
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DOI：
10.1073/pnas.100139797
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发表时间：
2000-06-06
影响因子：
11.1
通讯作者：
Halpain, S
中科院分区：
文献类型：
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作者：
Krucker, T;Siggins, GR;Halpain, S
动态肌动蛋白丝参与突触可塑性的特定方面的假说进行了研究在Schaffer侧支-CA 1锥体细胞突触的小鼠海马。低浓度（0.01-1 μ M）的化合物，抑制肌动蛋白丝组装浴应用于海马切片在胞外记录场兴奋性突触后电位。细胞松弛素D细胞松弛素B和latrunculin A都损害了短暂高频刺激诱导的LTP的维持。这种对LTP维持的作用是特异性的，因为没有一种化合物影响基础突触传递，成对脉冲易化。LTP诱导或强直后增强。细胞松弛素B的作用是可逆的。该结果与动态肌动蛋白丝在LTP早期维持阶段的分子机制中发挥重要作用的模型一致，例如新突触连接的生长或沉默突触的转换。
The hypothesis that dynamic actin filaments participate in specific aspects of synaptic plasticity was investigated at the Schaffer-collateral-CA1 pyramidal cell synapse of mouse hippocampus. Low concentrations (0.01-1 mu M) of compounds that inhibit actin filament assembly were bath applied to hippocampal slices during extracellular recording of field excitatory postsynaptic potentials. Cytochalasin D. cytochalasin B, and latrunculin A all impaired the maintenance of LTP induced by brief high-frequency stimulation. This effect on LTP maintenance was specific, because none of the compounds affected basal synaptic transmission, paired-pulse facilitation. LTP induction, or post-tetanic potentiation. The effect of cytochalasin B was reversible. The results are consistent with a model in which dynamic actin filaments play an essential role in the molecular mechanisms underlying the early maintenance phase of LTP, such as growth of new synaptic connections or conversion of silent synapses.