Efficient revascularization by VEGF administration via heparin-functionalized nanoparticle-fibrin complex
Efficient revascularization by VEGF administration via heparin-functionalized nanoparticle-fibrin complex
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DOI：
10.1016/j.jconrel.2010.01.010
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发表时间：
2010-05-10
影响因子：
10.8
通讯作者：
Kim, Young Ha
中科院分区：
文献类型：
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作者：
Chung, Yong-Il;Kim, Sang-Ki;Kim, Young Ha
我们研究了肝素功能化纳米颗粒-纤维蛋白凝胶复合物对血管内皮生长因子（VEGF）的血管生成生物活性和治疗血管生成作用。在小鼠皮下植入模型中，就毛细血管密度而言，与负载VEGF的纤维蛋白凝胶、不含VEGF的纳米颗粒-纤维蛋白凝胶复合物或纤维蛋白凝胶（对照）相比，负载VEGF的纳米颗粒-纤维蛋白凝胶复合物观察到显著增加的生物活性。此外，VEGF负载的纳米颗粒-纤维蛋白凝胶复合物显著增强了兔缺血后肢模型中的治疗性血管生成作用：与VEGF负载的纤维蛋白凝胶相比，恢复的小腿血压、血管造影评分和侧支密度显著增加，以及组织化侧支的稳定维持。这些结果表明，通过所提出的肝素官能化纳米颗粒-纤维蛋白凝胶复合物，VEGF施用的血管生成潜力增强。(C)2010 Elsevier B. V.保留所有权利。
We investigated the angiogenic bioactivity and therapeutic angiogenic effect of vascular endothelial growth factor (VEGF) administration by the heparin-functionalized nanoparticle-fibrin gel complex. The markedly increased bioactivity was observed by the VEGF-loaded nanoparticle-fibrin gel complex, compared to the VEGF-loaded fibrin gel, the nanoparticle-fibrin gel complex without VEGF, or fibrin gel (control) in terms of the capillary density in a mouse subcutaneous implantation model. Furthermore, the VEGF-loaded nanoparticle-fibrin gel complex significantly enhanced the therapeutic angiogenic effect in a rabbit ischemic hind limb model: the noticeable increase in the recovered calf blood pressure, the angiographic score, and the density of collaterals, as well as the stable maintenance of the organized collaterals, compared to the VEGF-loaded fibrin gel. These results show the enhanced angiogenic potential of VEGF administration by the proposed heparin-functionalized nanoparticle-fibrin gel complex. (C) 2010 Elsevier B.V. All rights reserved.