VIABILITY OF, AND TRANSFER OF A PLASMID FROM, ESCHERICHIA-COLI-K12 IN HUMAN INTESTINE
VIABILITY OF, AND TRANSFER OF A PLASMID FROM, ESCHERICHIA-COLI-K12 IN HUMAN INTESTINE
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DOI：
10.1038/255502a0
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发表时间：
1975-01-01
期刊：
影响因子：
64.8
通讯作者：
ANDERSON, ES
中科院分区：
文献类型：
--
作者：
ANDERSON, ES
关于大肠杆菌K 12能否在人类肠道中存活并将质粒转移到本地植物群的争论，是由于有人提议对细菌质粒和其他遗传因子进行研究，而这些遗传因子与来自与质粒正常宿主或质粒本身无关的生物体的DNA杂交。由于大多数微生物遗传学研究中使用的菌株伊势. coliK12，实验设计以研究其活力，和其质粒转移至宿主E的能力。大肠杆菌，在人类肠道中。我们向阿什比勋爵领导的工作组提交了我们的意见的初步摘要。
THE controversy over the ability ofEscherichia coliK12 to survive in the human gut and to transfer plasmids to the indigenous flora, arose in relation to proposed research on bacterial plasmids and other genetic agents hybridised with DNA from organisms unrelated to the normal hosts of the plasmids or to the plasmids themselves. As the bacterial strain used in most studies of microbial genetics isE. coliK12, experiments were designed to investigate its viability, and its capacity for plasmid transfer to the residentE. coli, in the human intestine. A preliminary summary of our observations was presented to the Working Party headed by Lord Ashby1.