In vitro attachment and invasion of chicken ovarian granulosa cells by Salmonella enteritidis phage type 8
In vitro attachment and invasion of chicken ovarian granulosa cells by Salmonella enteritidis phage type 8
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DOI：
10.1128/iai.64.12.5015-5021.1996
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发表时间：
1996-12
影响因子：
3.1
通讯作者：
D. Thiagarajan;M. Saeed;J. Turek;E. Asem
中科院分区：
文献类型：
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作者：
D. Thiagarajan;M. Saeed;J. Turek;E. Asem
在体外研究了肠炎沙门氏菌对鸡卵巢颗粒细胞的黏附和侵入。通过用抗鸡纤连蛋白抗体预孵育颗粒细胞（黏附减少约70%）或用从肠炎沙门氏菌分离的14 - kDa菌毛蛋白预孵育（黏附减少68%），黏附受到抑制。在将细菌细胞添加到颗粒细胞之前用四肽RGDS处理细菌细胞导致黏附受到抑制（当用500微克肽处理2×10⁷菌落形成单位的细菌时，抑制60%）。用肽GRGD处理导致相似程度的抑制，表明细胞外基质蛋白在肠炎沙门氏菌与颗粒细胞的相互作用中起重要作用。相反，用肽GRAD处理细菌细胞并未对其与颗粒细胞的黏附产生显著抑制。发现肠炎沙门氏菌特异性地黏附于纤连蛋白、Ⅳ型胶原蛋白和层粘连蛋白包被的微量滴定板孔，黏附的顺序如下：纤连蛋白＞层粘连蛋白＞Ⅳ型胶原蛋白。肠炎沙门氏菌侵入颗粒细胞的光镜和透射电镜照片显示，在颗粒细胞的细胞质中有带或不带周围膜的细菌。在某些情况下，在细胞质中观察到正在分裂的细菌细胞。这项研究的结果表明，肠炎沙门氏菌以特定的方式与颗粒细胞相互作用，并且能够侵入这些细胞并在其中繁殖。排卵前卵泡的颗粒细胞层可能是肠炎沙门氏菌侵袭株在鸡卵巢定植的优选部位。
The attachment and invasion of chicken ovarian granulosa cells by Salmonella enteritidis was examined in vitro. The attachment was inhibited by preincubation of granulosa cells with anti-chicken fibronectin antibody (approximately 70% reduction in attachment) or preincubation with a 14-kDa fimbrial protein isolated from S. enteritidis (68% reduction in attachment). Treatment of bacterial cells with the tetrapeptide RGDS before addition to granulosa cells resulted in inhibition of attachment (60% inhibition when 2 x 10(7) CFU of bacteria was treated with 500 microg of peptide). Treatment with the peptide GRGD resulted in similar magnitude of inhibition, indicating that extracellular matrix proteins play significant roles in the interaction of S. enteritidis with granulosa cells. In contrast, treatment of the bacterial cells with the peptide GRAD did not result in significant inhibition of attachment to the granulosa cells. S. enteritidis was found to attach specifically to fibronectin, collagen IV, and laminin-coated microtiter plate wells, with the rank order of attachment as follows: fibronectin > laminin > collagen IV. Light and transmission electron micrographs of S. enteritidis invasion of granulosa cells showed organisms with or without a surrounding membrane in the cytoplasm of granulosa cells. In some instances, dividing bacterial cells were observed in the cytoplasm. Results of this study demonstrated that S. enteritidis interacts with granulosa cells in a specific manner and can invade and multiply in these cells. The granulosa cell layer of the preovulatory follicles may be a preferred site for the colonization of the chicken ovaries by invasive strains of S. enteritidis.