A simple and inexpensive method for maintaining a defined flora mouse colony.
A simple and inexpensive method for maintaining a defined flora mouse colony.
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一种简单且廉价的维持特定菌群小鼠群体的方法。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
K. A. Mason
中科院分区：
文献类型：
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作者：
R. Sedlacek;K. A. Mason
在现有的小鼠群体中，使用高压灭菌的笼子、饲料、床上用品、水和滤帽，并结合无菌畜牧技术，在维持特定的菌群方面是无效的。增加了一个配备高效颗粒空气过滤器的层流空气流动实验台，提供了一个无菌环境，当过滤器盖被移除时，可以在其中操纵老鼠。安装了一条管道来引导所有通过台式过滤器进入房间的空气，减少了房间里空气中的细菌数量。这一改进与剔除或标记受感染的笼子相结合，使未来不会从这些笼子中带走繁殖者，从而消除了菌落中的一些细菌污染物(金黄色葡萄球菌、S表皮葡萄球菌和链球菌)。
The use of autoclaved cages, feed, bedding, water, and filter caps combined with aseptic techniques of animal husbandry in an existing mouse colony was ineffective in maintaining a defined flora colony. The addition of a laminar air flow bench equipped with a high efficiency particulate air filter provided a sterile environment in which to manipulate mice when the filter caps were removed. The installation of a duct to direct all air entering the room through the bench filter reduced the airborne bacterial counts in the room. This modification combined with the culling or marking of infected cages so that no future breeders would be taken from these cages eliminated a number of bacterial contaminants (Staphylococcus aureus, S epidermidis, and streptococci) from the colony.