Developmental switch in chromatin structure associated with alternate promoter usage in the Drosophila melanogaster alcohol dehydrogenase gene.
Developmental switch in chromatin structure associated with alternate promoter usage in the Drosophila melanogaster alcohol dehydrogenase gene.
复制标题
染色质结构的发育转变与果蝇乙醇脱氢酶基因中替代启动子的使用相关。
DOI：
10.1002/j.1460-2075.1987.tb02618.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Cartwright,IL
中科院分区：
文献类型：
--
作者：
Cartwright,IL
在黑腹果蝇的发育过程中，酒精脱氢酶基因启动子的使用发生了变化，使得近端启动的mRNA被远端启动的mRNA所取代。在Adh表达不活跃的细胞中，或来自近端或远端启动子活跃的组织中，对该基因的核蛋白组织的研究揭示了核小体组织模式和核酸酶敏感区域的明显变化，这些变化与该基因的活性及其启动子的使用密切相关。定位的核小体阵列覆盖无活性基因的编码区，但在基因激活时部分解体。在早期三龄幼虫脂肪体细胞中检测到的一系列近端超敏部位，在晚期三龄幼虫脂肪体中被新的远端超敏区域所取代，这种变化显然与启动子开关相一致。在远端起始位点上游超过1kb的区域中发现了进一步的发育阶段差异。此外，对于近端和远端表达细胞，在染色质中分离的和不同的明显抵抗dna酶I切割的区域被检测到，在某些情况下，在体外被证明可以结合特定因子。
During the development of Drosophila melanogaster a switch in alcohol dehydrogenase gene promoter usage occurs, such that proximally initiated mRNA is replaced by mRNA initiated from a more distal location. Investigation of the nucleo‐protein organization at this gene in cells inactive for Adh expression, or derived from tissues active at either the proximal or distal promoter, reveals distinct changes in patterns of nucleosome organization and regions of nuclease sensitivity that are strongly correlated with the activity of the gene and its promoter usage. A positioned array of nucleosomes covers the coding region of the inactive gene, but is partially disassembled on gene activation. A series of proximally located hypersensitive sites, detected in early third instar larval fat body cells, are replaced by new, distally located regions of hypersensitivity in late third instar larval fat body, the change apparently coinciding with the promoter switch. Further developmental stage differences are detected in regions over 1 kb upstream of the distal start site. In addition, for both proximally and distally expressing cells, separate and different regions of apparent resistance to DNase I cleavage in chromatin are detected in locations that, in some instances, were previously demonstrated to bind specific factors in vitro.