Serological evidence of Zika virus infection in non-human primates in Zambia
Serological evidence of Zika virus infection in non-human primates in Zambia
复制标题
赞比亚非人类灵长类动物感染寨卡病毒的血清学证据
DOI：
10.1007/s00705-019-04302-0
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发表时间：
2019
影响因子：
2.7
通讯作者：
Hirofumi Sawa
中科院分区：
文献类型：
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作者：
Christida E. Wastika;Michihito Sasaki;Kentaro Yoshii;Paulina D. Anindita;Bernard M. Hang’ombe;Aaron S. Mweene;Shintaro Kobayashi;Hiroaki Kariwa;Michael J. Carr;William W. Hall;Yuki Eshita;Yasuko Orba;Hirofumi Sawa
寨卡病毒(ZIKV)在非人灵长类动物(HHP)之间以平坦的传播周期传播。为了调查赞比亚NHP中黄病毒感染的证据，我们进行了空斑减少中和试验(PRNT)以定量中和抗体。结果表明，NHP血清对ZIKV有中和活性(34.4%；33/96)，而用NHP血清对黄热病病毒无中和活性。在非霍奇金淋巴瘤患者的脾组织中未检测到ZIKV基因组RNA，这表明抗ZIKV中和抗体的存在代表已解决的感染。我们的证据表明，ZIKV存在于赞比亚的NHP水库中。
Zika virus (ZIKV) circulation occurs between non-human primates (NHPs) in a sylvatic transmission cycle. To investigate evidence of flavivirus infection in NHPs in Zambia, we performed a plaque reduction neutralization test (PRNT) to quantify neutralizing antibodies. PRNT revealed that sera from NHPs (African green monkeys and baboons) exhibited neutralizing activity against ZIKV (34.4%; 33/96), whereas a PRNT for yellow fever virus using NHP sera showed no neutralization activity. ZIKV genomic RNA was not detected in splenic tissues from NHPs, suggesting that the presence of anti-ZIKV neutralizing antibodies represented resolved infections. Our evidence suggests that ZIKV is maintained in NHP reservoirs in Zambia.