Ovulation in adult rats after treatment with pregnant mare serum gonadotrophin during oestrus.
Ovulation in adult rats after treatment with pregnant mare serum gonadotrophin during oestrus.
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成年大鼠在发情期间用孕马血清促性腺激素治疗后的排卵。
DOI：
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发表时间：
1971
期刊：
影响因子：
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通讯作者：
M. Rutte
中科院分区：
文献类型：
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作者：
R. Welschen;M. Rutte
通过测量正常大鼠和去垂体大鼠的卵泡数、卵泡大小和卵子数，分析了孕马血清促性腺激素(PMS)和人绒毛膜促性腺激素(HCG)诱导的大鼠“超数排卵”。在正常大鼠和发情期应用HCG的大鼠中，5500万立方米或更大的卵泡自发排卵。5-50IU PMS可使大卵泡数增加到20-60个/只，但在下午2点注射15-45IU HCG，1-4天后排卵量减少，卵泡保留一半。5、10或50IU经前综合征(无HCG)在3或4天后(正常发情后5天)诱发自发排卵。这些给予10或50IU PMS的大鼠在排卵后第二天出现黄素化的囊性卵泡并包裹卵子。在发情期给予10-35IU PMS的大鼠不排卵，而给予50-80IU PMS的大鼠仅释放少量卵子。为估计内源性促黄体生成素的释放量，在不同时间切除大鼠脑垂体，给予不同剂量的促性腺激素。切除垂体的实验结果表明，经前综合征后5或50~80IU的内源性黄体生成素是充足的，但在10~35IU后内源性黄体生成素低于正常。
"Superovulation" in rats induced by treatment with pregnant mare serum gonadotropin (PMS) and human chorionic gonadotropin (HCG) was analyzed by measuring numbers and size of follicles, and number of ova, in normal and hypophysectomized rats given these hormones. In normal rats and in rats treated with HCG during estrus, follicles of 55,000,000 cubic mcm or larger ovulate spontaneously. 5-50 IU PMS accelerated development of increased numbers of large follicals to 20-60 per rat, but injection of 15-45 IU HCG at 2 pm, 1-4 days later permitted few ovulations and left half the follicles retained. 5, 10, or 50 IU PMS (no HCG) evoked spontaneous ovulation 3 or 4 days later (5 days after estrus is normal). These rats given 10 or 50 IU PMS had cystic follicles with luteinized walls and entrapped ova, on the day after ovulation. Rats given 10-35 IU PMS in estrus did not ovulate, and those given 50-80 IU PMS released only a few ova. As an estimate of the amount of endogenous LH released, rats were hypophysectomized at various times and given various doses of HCG. Results of hypophysectomy experiments suggested that endogenous LH is sufficient after 5 or 50-80 IU PMS, but is subnormal after 10-35 IU.