Over-expression of an S-adenosylmethionine-dependent methyltransferase leads to an extended lifespan of Podospora anserina without impairments in vital functions
Over-expression of an S-adenosylmethionine-dependent methyltransferase leads to an extended lifespan of Podospora anserina without impairments in vital functions
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DOI：
10.1111/j.1474-9726.2008.00412.x
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发表时间：
2008-10-01
期刊：
影响因子：
7.8
通讯作者：
Osiewacz, Heinz D.
中科院分区：
文献类型：
--
作者：
Kunstmann, Birgit;Osiewacz, Heinz D.
PaMTH1是一种可能的甲基转移酶，先前被描述为在鹅绒泡霉衰老过程中总蛋白提取物的丰度增加，已证明在衰老培养物的线粒体细胞部分中积累。该蛋白定位于线粒体基质中，并显示出利用类黄酮作为底物的甲基转移酶活性。PaMth1在P.anserina中的结构性过表达减少了蛋白质的羰基化，延长了寿命，而不损害生命功能，这表明PaMTH1对氧化应激具有保护作用。
PaMTH1, a putative methyltransferase previously described to increase in abundance in total protein extracts during aging of Podospora anserina is demonstrated to accumulate in the mitochondrial cell fraction of senescent cultures. The protein is localized in the mitochondrial matrix and displays a methyltransferase activity utilizing flavonoids as substrates. Constitutive over-expression of PaMth1 in P. anserina results in a reduced carbonylation of proteins and an extended lifespan without impairing vital functions suggesting a protecting role of PaMTH1 against oxidative stress.