Hemoglobin equilibrium analysis by the multiangle laser light-scattering method.
Hemoglobin equilibrium analysis by the multiangle laser light-scattering method.
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DOI：
10.1006/bbrc.2002.6362
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发表时间：
2002-02
影响因子：
3.1
通讯作者：
Takamasa Yamaguchi;K. Adachi
中科院分区：
文献类型：
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作者：
Takamasa Yamaguchi;K. Adachi
采用微批次多角度光散射（MALS）分析系统，分别对四聚血红蛋白及其单链在CO形式下的二聚体-四聚体和单体-二聚体-四聚体平衡进行了评价。人血红蛋白A和血红蛋白F在CO形式的分子量依赖于浓度。Hb A和Hb F二聚体的解离常数分别为2.58 × 10 ~（-6）和0.66 × 10 ~（-6）。还通过相同的方法评价了单个珠蛋白链（包括α、β和γ链）的平衡。α链二聚体与单体、β链四聚体与单体、γ链四聚体与二聚体的解离常数分别为14 × 10 - 6、25 × 10 - 17和6.86 × 10 - 6 M。这些结果表明，MALS分析系统不仅可以确定分子量，而且可以表征多亚基蛋白质的蛋白质-蛋白质相互作用。
Dimer-tetramer and monomer-dimer-tetramer equilibria of tetrameric hemoglobins and their single chains in the CO form, respectively, were evaluated using the microbatch multiangle light-scattering (MALS) analysis system. The molecular weights of human Hb A and Hb F in the CO form were dependent on concentration. The dissociation constants to dimers of Hb A and Hb F were 2.58 x 10(-6) and 0.66 x 10(-6), respectively. Equilibration of single globin chains, including alpha, beta, and gamma chains, was also evaluated by the same method. The dissociation constants of alpha-chain dimers to monomers, of beta-chain tetramers to monomers, and of gamma-chain tetramers to dimers were 14 x 10(-6), 25 x 10(-17), and 6.86 x 10(-6) M, respectively. These results indicate that the MALS analysis system can not only determine molecular weight but also characterize protein-protein interactions of multi-subunit proteins.