Rehmannia glutinosa polysaccharide induced an anti-cancer effect by activating natural killer cells
Rehmannia glutinosa polysaccharide induced an anti-cancer effect by activating natural killer cells
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地黄多糖通过激活自然杀伤细胞诱导抗癌作用
DOI：
10.1016/j.ijbiomac.2017.07.090
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发表时间：
2017
影响因子：
8.2
通讯作者：
Jin Jun-O
中科院分区：
文献类型：
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作者：
Xu Li;Zhang Wei;Zeng Ling;Jin Jun-O
地黄glutinosapolysaccharide （RGP）具有免疫刺激作用，如激活树突状细胞，但在小鼠体内对自然杀伤细胞（NK）的激活能力方面还没有研究。在这项研究中，我们检测了RGP对脾、肠系膜淋巴结（mLN）和血液中NK细胞增殖和活化的影响，并将其功能与大肠杆菌多糖的功能进行了比较。给C57BL/6小鼠RGP可诱导循环血液中NK细胞数量增加，脾脏、mLN和血液中NK细胞增殖增加。此外，RGP治疗促进了脾脏NK细胞中toll样受体-4依赖性干扰素γ的产生和CD69表达的上调。rgp处理的NK细胞通过产生I型IFN来增强对目标Yac-1细胞的细胞毒活性。最后，RGP治疗抑制了CT26肿瘤在肺部的生长。这些结果表明，RGP在小鼠体内促进NK细胞的活化，抑制肿瘤的生长。
Rehmannia glutinosapolysaccharide (RGP) has exhibited an immune stimulatory effect, such as dendritic cell activation, but it has not been studied in terms of the activation capacity of natural killer (NK) cells in micein vivo. In this study, we examined the effect of RGP on the proliferation and activation of NK cells in the spleen, mesenteric lymph node (mLN), and bloodin vivoand compared that function with that ofEscherichia colilipopolysaccharide. The administration of RGP to C57BL/6 mice induced the increase in circulating blood NK cell numbers and in the proliferation of NK cells in the spleen, mLN, and blood. Moreover, RGP treatment promoted the toll-like receptor-4-dependent production of interferon-gamma and the up-regulation of CD69 expression in spleen NK cells. RGP-treated NK cells enhanced the cytotoxic activity with type I IFN production against target Yac-1 cells. Finally, RGP treatment inhibited the CT26 tumor growth in the lung. These findings demonstrated that RGP promoted the activation of NK cells and inhibited tumor growth in micein vivo.