Prognostic factors in MNU and DMBA-induced mammary tumors in female rats
Prognostic factors in MNU and DMBA-induced mammary tumors in female rats
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DOI：
10.1016/j.prp.2017.02.014
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发表时间：
2017-01-01
影响因子：
2.8
通讯作者：
Colaco, Bruno
中科院分区：
文献类型：
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作者：
Alvarado, Antonieta;Lopes, Ana C.;Colaco, Bruno
由致癌物 1-甲基-1-亚硝基脲 (MNU) 和 7,12-二甲基苯并[a]蒽 (DMBA) 化学诱发的大鼠乳腺肿瘤是人类乳腺癌相关研究中最广泛使用的模型。本研究旨在评估MNU和DMBA诱导的大鼠乳腺肿瘤中雌激素受体α（ERα）、孕激素受体（PR）和Ki-67等预后因素的免疫表达，以了解最适合女性乳腺癌的模型。对 28 个 MNU 诱导的和 16 个 DMBA 诱导的处女雌性 Sprague-Dawley 大鼠乳腺肿瘤进行了分析。通过免疫组织化学评估预后标志物 ERα、PR 和 Ki-67 增殖指数 (Ki-67 PI) 的表达。还评估了有丝分裂活性指数（MAI）。在每个乳腺肿瘤中鉴定出不止一种组织学模式。癌构成了两种致癌物最常诱发的病变：33 种 MNU 诱发的癌和 23 种 DMBA 诱发的癌。所有MNU和DMBA诱导的乳腺癌均为ER+/PR+，与PR相比，ERα的表达更高。与DMBA诱导的乳腺癌相比，MNU诱导的乳腺癌的肿瘤重量、ERα、PR、Ki-67PI和MAI的表达较高。 ER α、PR 和 MAI 观察到组间有统计学显着性差异 (p
Chemically-induced mammary tumors in rats by the carcinogens 1-methyl-1-nitrosourea-(MNU) and 7,12-dimethylbenz[a]anthracene (DMBA) are the most widely used models for studies related with human breast cancer. This study aimed to evaluate the immunoexpression of the prognostic factors estrogen receptor alpha (ER alpha), progesterone receptor (PR) and Ki-67, in MNU and DMBA-induced rat mammary tumors, in order to know the model that best suits to woman breast cancer. Twenty-eight MNU-induced and 16 DMBA-induced mammary tumors in virgin female Sprague-Dawley rats were analyzed. The expression of the prognostic markers ER alpha, PR and Ki-67 proliferation index (Ki-67 PI) was assessed by immunohistochemistry. Mitotic activity index (MAI) was also evaluated. More than one histological pattern was identified in each mammary tumor. Carcinomas constituted the lesions most frequently induced by both carcinogens: 33 MNU-induced carcinomas and 23 DMBA-induced carcinomas. All MNU and DMBA-induced mammary carcinomas were ER+/PR+, with a higher expression of ER alpha when compared with PR. Tumors' weight, the expression of ER alpha, PR, Ki-67 PI and MAI were higher in MNU-induced mammary carcinomas when compared with the DMBA-induced ones. Statistically significant differences between groups were observed for ER alpha, PR and MAI (p