Prevalence of antibiotic resistance genes in bacteriophage DNA fraction from Funan River water in Sichuan, China
Prevalence of antibiotic resistance genes in bacteriophage DNA fraction from Funan River water in Sichuan, China
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中国四川府南河水噬菌体 DNA 片段中抗生素抗性基因的流行率
DOI：
10.1016/j.scitotenv.2018.01.148
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发表时间：
2018
影响因子：
9.8
通讯作者：
Zhang Anyun
中科院分区：
文献类型：
--
作者：
Yang Yanxian;Shi Wenjin;Lu Shao Yeh;Liu Jinxin;Liang Huihui;Yang Yifan;Duan Guowei;Li Yunxia;Wang Hongning;Zhang Anyun
To better understand the role that bacteriophages play in antibiotic resistance genes (ARGs) dissemination in the aquatic environment, 36 water samples were collected from the Funan River in Sichuan, China. The occurrence of 15 clinically relevant ARGs and one class 1 integron geneint1in phage-particle DNA were evaluated by PCR. The abundance of ARGs (blaCTX-M,sul1, andaac-(6′)-1b-cr) was determined by quantitative PCR (qPCR). High prevalence of theint1gene (66.7%) was found in the phage-particle DNA of tested samples, followed bysul1(41.7%),sul2(33.3%),blaCTX-M(33.3%),aac-(6′)-lb-cr(25%),aph(3′)-IIIa (16.7%), andermF(8.3%). The qPCR data showed higher gene copy (GC) numbers in samples collected near a hospital (site 7) and a wastewater treatment plant (WWTP) (site 10) (P< .05). Particularly the absolute abundance ofaac-(6′)-lb-crgene was significantly higher than theblaCTX-Mandsul1genes with the gene copy (GC) numbers of 5.73 log10copy/mL for site 7 and 4.99 log10copy/mL for site 10. To our best knowledge, this is the first study to report the presence ofsul2,aac-(6′)-lb-cr,ermFandaph(3′)-IIIagenes in bacteriophage DNA derived from aquatic environments. Our findings highlight the potential of ARGs to be transmitted via bacteriophages in the aquatic environment.
