THE GENOME OF MINUTE VIRUS OF MICE, AN AUTONOMOUS PARVOVIRUS, ENCODES 2 OVERLAPPING TRANSCRIPTION UNITS
THE GENOME OF MINUTE VIRUS OF MICE, AN AUTONOMOUS PARVOVIRUS, ENCODES 2 OVERLAPPING TRANSCRIPTION UNITS
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DOI：
10.1093/nar/11.4.1019
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发表时间：
1983-01-01
影响因子：
14.9
通讯作者：
ASTELL, CR
中科院分区：
文献类型：
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作者：
PINTEL, D;DADACHANJI, D;ASTELL, CR
在感染小鼠细小病毒（MVM）的鼠细胞中已鉴定出四种病毒特异性转录物。这些RNA的长度分别为4.8、3.3、3.0和1.8个内切酶，分别命名为R1至R4，它们都是从DNA的病毒体（-）链转录的，并且它们都是聚腺苷酸化和剪接的。Rl转录物来源于位于基因组上4.0至95个图谱单位（mu）之间的序列。转录本R2由来自μ坐标4.0-10.0、40-46和48-95的外显子序列组成。最丰富的RNA，R3，是从40和95 μ之间的序列映射转录。所有这三种RNA都具有46-48 μ之间的短内含子序列。丰度最低的转录本R4尚未被精确定位，但它与跨越整个5 kb基因组的所有三个EcoRI片段杂交，克隆的MVM DNA限制性片段的体外转录证实了在图坐标4.0和39处存在功能性启动子，对病毒DNA的这些区域的序列分析揭示了RNA聚合酶II启动子的特征。这些结果表明，MVM DNA编码两个重叠的转录单位与单独的启动子附近的左端（4.0亩）和中间（39亩）的基因组。
Four virus–specific transcripts have been identified in murine cells infected with Minute–Virus–of–Mice (MVM). These RNAs, 4.8, 3.3, 3.0 and 1.8 kilobases in length, designated Rl to R4 respectively, are all transs–cribed from the virion (−) strand of DNA and they are all polyadenylated and spliced. The Rl transcript is derived from sequences that reside on the genome between 4.0 and 95 map units (mu). Transcript R2 is composed of exon sequences derived from mu coordinates 4.0–10.0, 40–46 and 48–95. The most abundant RNA, R3, is transcribed from sequences mapping between 40 and 95 mu. All three of these RNAs have a short intron sequence between 46–48 mu removed. The least abundant transcript, R4, has not been mapped precisely, however it hybridizes with all three EcoRI fragments which span the entire 5 kb genome.Invitrotranscription of cloned restriction fragments of MVM DNA confirm the existence of functional promoters at map coordinates 4.0 and 39 and sequence analysis of these regions of the viral DNA reveal the characteristic features of RNA polymerase II promoters. These results indicate that MVM DNA encodes two overlapping transcription units with separate promoters near the left end (4.0 mu) and middle (39 mu) of the genome.