Immunohistochemical demonstration of marked changes in the LHRH system of photosensitive and photorefractory European starlings (Sturnus vulgaris).
Immunohistochemical demonstration of marked changes in the LHRH system of photosensitive and photorefractory European starlings (Sturnus vulgaris).
复制标题
免疫组织化学证明光敏性和光难治性欧洲八哥 (Sturnus vulgaris) 的 LHRH 系统发生显着变化。
DOI：
10.1677/joe.0.1150211
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Brian K. Follett
中科院分区：
文献类型：
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作者：
Russell G. Foster;G. Plowman;Arthur R. Goldsmith;Brian K. Follett
采用免疫细胞化学方法研究了光周期对雄性欧椋鸟大脑LHRH神经分泌系统的影响。和其他鸟类一样，这种鸟类的繁殖是由长时间的日照触发的，但持续的日照会导致光难性，并导致生殖系统完全关闭。这些影响被认为是通过LHRH分泌的变化介导的。在暴露于11小时光照：13小时黑暗（11L:13D）和睾丸发育完全的椋鸟中，LHRH外核（n = 522 +/- 43 S.E.M.）和纤维都有很强的免疫染色。睾丸未发育的光敏短日（8L:16D）椋鸟具有几乎相同的强免疫反应性核周分布（n = 523 +/- 62），但纤维较少。在正中隆起处，纤维数量显著减少约30% （P < 0.01）。在长日照（18L:6D）、睾丸完全退化的光抗性椋鸟中，LHRH系统的变化更为明显。微核细胞的免疫反应较弱，平均直径从10微米减少到6.5微米（P < 0.01）。此外，细胞数量显著减少（P < 0.05）（312 +/- 62），尽管这可能是由于一些弱染色细胞低于分辨率限制而无法计数的结果。LHRH纤维几乎完全从正中隆起处消失，在大脑的其他部位也看不见。长时间光照诱导的光耐性调控的高级神经通路尚不清楚，但很明显核周LHRH的产生和末端LHRH的释放/储存正在被深刻地改变。
Immunocytochemistry was used to determine the effects of photoperiod on the LHRH neurosecretory system in the brain of male European starlings. In this species, as in other birds, reproduction is triggered by long daylengths but continued exposure leads to photorefractoriness and to a complete shut-down of the reproductive system. These effects are thought to be mediated through changes in the secretion of LHRH. In starlings exposed to a photoperiod of 11 h light:13 h darkness (11L:13D) and with fully developed testes there was strong immunostaining of both LHRH perikarya (n = 522 +/- 43 S.E.M.) and fibres. Photosensitive short-day (8L:16D) starlings with undeveloped testes had an almost identical distribution of strongly immunoreactive perikarya (n = 523 +/- 62) but there were fewer fibres. In the median eminence, fibre number was reduced significantly (P less than 0.01) by some 30%. In long-day (18L:6D) photorefractory starlings with fully regressed testes there was an even more obvious change in the LHRH system. Perikarya were only weakly immunoreactive and there was a significant (P less than 0.01) reduction in mean diameter from 10 to 6.5 micron. In addition, there was a significant (P less than 0.05) reduction in cell number (312 +/- 62), although this may well result from the fact that some weakly stained cells fell below the limits of resolution and could not be counted. LHRH fibres disappeared almost entirely from the median eminence, and were not visible elsewhere in the brain. The higher neural pathways regulating photorefractoriness induced by long days are unknown but clearly both production of LHRH in the perikarya and release/storage of LHRH in the terminals is being profoundly modified.