Markers of cell activation and apoptosis in bone marrow mononuclear cells of patients with autoimmune hepatitis type 1 and primary biliary cirrhosis
Markers of cell activation and apoptosis in bone marrow mononuclear cells of patients with autoimmune hepatitis type 1 and primary biliary cirrhosis
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DOI：
10.1016/j.jhep.2004.11.023
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发表时间：
2005-03-01
影响因子：
25.7
通讯作者：
Dalekos, GN
中科院分区：
文献类型：
--
作者：
Tsikrikoni, A;Kyriakou, DS;Dalekos, GN
背景/目标：我们报道了骨髓（BM）祖细胞在自身免疫性肝炎1型（AIH-1）和原发性胆汁性肝硬化（PBC）的定量和定性差异。本研究探讨了AIII-I和PBC患者骨髓单个核细胞（BMMCs）长期培养中的细胞凋亡特征和造血抑制因子。方法：检测13例AIII-I患者、13例PBC患者、12例正常对照和10例健康对照骨髓单个核细胞（BMMCs）的凋亡标志物和CD 14表达。结果：与对照组相比，AIII-I组和PBC组的所有凋亡标志物和CD 14均升高（P < 0.0001）。AIH-1和PBC中Fas +细胞与凋亡细胞呈正相关（P = 0.0001）。与对照组相比，AIH-1（P = 0.003和P = 0.001）和PBC（P = 0.0001）中的TNF-α和IFN-γ较高。对照groups.Conclusions之间没有发现差异：我们第一次证明，从AIII-I和PBC患者的骨髓基质细胞中的细胞凋亡过程，巨噬细胞活化和造血细胞因子抑制剂的生产是高于对照组。Fas-FasL途径可能参与凋亡过程;所选细胞因子水平的增加可能有助于Fas-FasL刺激。肝硬化似乎不太可能是上述发现的原因。(c)2004年欧洲肝脏研究协会。Elsevier B. V.出版，保留所有权利。
Background/Aims: We have reported quantitative and qualitative differences in bone marrow (BM) progenitor cells in autoimmune hepatitis type-1 (AIH-1) and primary biliary cirrhosis (PBC). This study investigated the apoptotic features and cytokine suppressors of haematopoiesis in long-term cultures of BM mononuclear cells (BMMCs) from AIII-I and PBC patients.Methods: Apoptotic markers and CD14 expression were evaluated in 13 AIII-I patients, 13 PBC patients, 12 cirrhotic controls and 10 healthy subjects. TNF-alpha, TGF-beta and IFN-gamma were determined using ELISAs.Results: All apoptotic markers and CD14 were increased in AIII-I and PBC compared to controls (P < 0.0001). Fas + cells were positively correlated (P = 0.0001) with apoptotic cells in AIH-1 and PBC. TNF-alpha and IFN-gamma were higher in AIH-1 (P = 0.003 and P = 0.001) and PBC (P = 0.0001) compared to controls. No differences were found between the control groups.Conclusions: We demonstrate for the first time that the apoptotic process, macrophage activation and the production of cytokine suppressors of haematopoiesis in BMMCs from AIII-I and PBC patients are higher compared to controls. The Fas-FasL pathway is likely to be involved in the apoptotic process; the increased levels of selected cytokines may contribute to Fas-FasL stimulation. Cirrhosis appears unlikely to be the cause of the above findings. (c) 2004 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.