Demonstrating circulation in vasculogenic mimicry patterns of uveal melanoma by confocal indocyanine green angiography
Demonstrating circulation in vasculogenic mimicry patterns of uveal melanoma by confocal indocyanine green angiography
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DOI：
10.1038/sj.eye.6702783
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发表时间：
2008-07-01
期刊：
影响因子：
3.9
通讯作者：
Pe'er, J.
中科院分区：
文献类型：
--
作者：
Frenkel, S.;Barzel, I.;Pe'er, J.
目的：血管生成拟态是由高度侵袭性的黑色素瘤细胞形成的，在体内外与内皮细胞衬里的血管相连，并含有液体。本研究的目的是确定是否液体泄漏到血管生成拟态模式没有循环，或者如果流体循环和清除这些patterns.Methods吲哚菁绿色（ICG）激光扫描共聚焦血管造影（海德堡视网膜血管造影仪（HRA），海德堡工程，海德堡，德国）进行了9例后脉络膜黑色素瘤在机构设置。在ICG注射之前和从ICG注射部位的对侧臂以及注射后1分钟抽取血液。结果测量包括视网膜血管和血管生成拟态模式首次充盈的时间以及HRA仪器检测不到荧光的时间。荧光不再检测到血管或模式后，管中含有患者的血液进行成像的海德堡HRA.Results循环血管生成拟态模式检测局灶性在5名患者注射后30 s内，并可检测到注射后12 min。在ICG注射前抽取的血液没有自发荧光，但ICG含有血液汇集在管中继续荧光在1个月postinjection.Conclusions血管生成拟态模式的内皮细胞内衬的血管系统和流体进入这些模式通过泄漏。注射后ICG快速输注到这些模式中以及海德堡HRA可检测到的荧光消失表明液体在这些模式中循环，并且不会积聚为停滞池。
Purpose Vasculogenic mimicry patterns, formed by highly invasive melanoma cells, connect to endothelial cell-lined blood vessels and contain fluid in vitro and in vivo. This study was designed to determine if fluid leaks into vasculogenic mimicry patterns without circulation, or if fluid circulates in and clears from these patterns.Methods Indocyanine green (ICG) laser scanning confocal angiography (Heidelberg Retinal Angiograph (HRA); Heidelberg Engineering, Heidelberg, Germany) was performed on nine patients with posterior choroidal melanoma in an institutional setting. Blood was drawn before the ICG injection and from the contralateral arm of the ICG injection site and 1 min after the injection. Outcome measures include time to first filling of retinal vessels and vasculogenic mimicry patterns and the time at which no fluorescence could be detected by the HRA instrument. After fluorescence was no longer detected in vessels or patterns, the tubes containing the patient's blood was imaged by the Heidelberg HRA.Results Looping vasculogenic mimicry patterns were detected focally in five patients within 30 s after injection and were detectable up to 12 min post-injection. Blood drawn before ICG injection did not autofluoresce but ICG-containing blood pooled in the tube continued to fluoresce at 1-month postinjection.Conclusions Vasculogenic mimicry patterns are not part of the endothelial cell-lined vascular system and fluid enters these patterns through leakage. The rapid infusion of ICG into these patterns after injection and the disappearance of fluorescence detectable by the Heidelberg HRA suggest that fluid circulates in these patterns and does not accumulate as a stagnant pool.