An immunohistological study of cytokeratin 20 in human and mammalian oral epithelium
An immunohistological study of cytokeratin 20 in human and mammalian oral epithelium
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DOI：
10.1016/s0003-9969(00)00050-9
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发表时间：
2000-10-01
影响因子：
3
通讯作者：
Berkovitz, BKB
中科院分区：
文献类型：
--
作者：
Barrett, AW;Cort, EM;Berkovitz, BKB
细胞角蛋白（CK）20是一种低分子量中间丝，据报道仅由良性和恶性胃肠道上皮、尿路上皮和默克尔细胞表达。本文的主要目的是绘制其在人类和其他哺乳动物正常口腔粘膜中的表达，并确定其是否在异常人类口腔上皮中表达。唾液和牙源性上皮也进行了分析。免疫过氧化物酶法用于石蜡包埋和冷冻切片。此外，进行双标记实验以确定CK 20表达与CK 8/18或S 100蛋白表达之间的关联。正常人口腔粘膜从四个网站，连同腹部皮肤，进行了研究，从32个人的尸检样本。在下颌牙龈、硬腭、颊粘膜、舌侧缘和腹部皮肤中，分别有24/32（75.0%）、25/32（78.1%）、7/32（21.9%）、0/32（0/32）和2/32（6.3%）的标本中检测到与默克尔细胞一致的CK 20阳性基底部圆形或角形细胞。双标记显示所有CK 20阳性的默克尔细胞也表达CK 8/18和S 100。唯一表达CK 20的细胞是人类味蕾。活检样本中不典型增生或浸润性口腔上皮细胞无表达。人、绒猴、雪貂、兔和豚鼠的结肠粘膜管腔细胞呈阳性，而非人种的口腔粘膜普遍呈阴性。结论：口腔粘膜中CK 20是默克尔细胞和味蕾的特异性标志物，默克尔细胞在角化的口腔粘膜中比在非角化的口腔粘膜中更常见，CK 20阳性的默克尔细胞也是S100阳性的，CK 20多肽组成可能存在种间差异，并且与尿路上皮相比，CK 20对口腔上皮异常增生的诊断价值不大。(C)2000爱思唯尔科技有限公司版权所有。
Cytokeratin (CK) 20 is a low molecular-weight intermediate filament reportedly expressed only by benign and malignant gastrointestinal epithelium, urothelium and Merkel cells. The main aims here were to map its expression in normal oral mucosa of humans and other mammals, and to determine whether it was expressed by abnormal human oral epithelium. Salivary and odontogenic epithelium were also analysed. An immunoperoxidase method was used on wax-embedded and cryostat sections. In addition, double-labelling experiments were undertaken to determine the association between CK 20 expression and that of CK 8/18 or S100 protein. Normal human oral mucosa from four sites, together with abdominal skin, was studied in autopsy samples from 32 individuals. CK 20-positive, basally situated, round or angular cells, consistent with Merkel cells, were recorded in 24/32 (75.0%) samples of mandibular gingiva, 25/32 (78.1%) samples of hard palate, 7/32 (21.9%) samples of buccal mucosa, 0/32 samples of lateral border of tongue, and 2/32 (6.3%) samples of abdominal skin. Double-labelling showed that all CK 20-positive Merkel cells also expressed CK 8/18 and S100. The only other cells to express CK 20 were human taste buds. There was no expression by dysplastic or invasive oral epithelium from biopsy samples. Colonic mucosa showed luminal-cell positivity in man, marmoset, ferret, rabbit and guinea-pig, but oral mucosa was universally negative in non-human species. It is concluded that in oral mucosa CK 20 is a specific marker of Merkel cells and taste buds, that Merkel cells are more frequently present in keratinized than non-keratinized oral mucosa, that CK 20-positive Merkel cells are also S100-positive, that there may be interspecies variations in CK 20 polypeptide composition and that, by contrast to urothelium, CK 20 has no value in the diagnosis of oral epithelial dysplasia. (C) 2000 Elsevier Science Ltd. All rights reserved.