Evaluating yeast biosynthetic vacuolar transport.
Evaluating yeast biosynthetic vacuolar transport.
复制标题
评估酵母生物合成液泡运输。
DOI：
10.1007/978-1-59745-466-7_22
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Horazdovsky,BruceF
中科院分区：
文献类型：
--
作者：
Davies,BrianA;Carney,DarrenS;Horazdovsky,BruceF
Study of the lysosomal protein transport system has been facilitated through dissection of the analogous vacuolar protein sorting (VPS) pathway inSaccharomyces cerevisiae. Resident enzymes of the yeast vacuole are synthesized as inactive precursors and are cleaved to their mature forms upon delivery to this compartment. Quantitative assessment of this delivery can be achieved through the use of pulse-chase experiments monitoring the cleavage of zymogens to their mature forms. The experimental procedures for analysis of carboxypeptidase Y (CPY) and carboxypeptidase S (CPS) maturation are described.
