POLYMORPHISM OF HUMAN-ERYTHROCYTE C3B C4B RECEPTOR
POLYMORPHISM OF HUMAN-ERYTHROCYTE C3B C4B RECEPTOR
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DOI：
10.1073/pnas.80.6.1698
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
ATKINSON, JP
中科院分区：
文献类型：
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作者：
DYKMAN, TR;COLE, JL;ATKINSON, JP
人红细胞补体C3、C4组分主要激活片段受体(hUE-C3bR)是从个体献血者中分离得到的。红细胞表面用~(125)I标记，并用NONIDT P-40增溶。用C3-琼脂糖亲和层析纯化HUE-C3bR，并用NaDodSO4/聚丙烯酰胺凝胶放射自显影进行分析。我们展示了三种截然不同的受体模式。类型A有一个主带，其相对分子质量为190,000。类型b有一条主带，分子量为220,000，类型c有两条主带，分子量为190,000和220,000。在所有三种类型中，通常在比每个主要带大15,000兆瓦的地方观察到一个次要带，占总放射性的25%。用甲胺灭活的C4-琼脂糖亲和层析或用抗HUE-C3bR的单抗免疫沉淀可获得相同的放射自显影图谱。还原和烷基化后，三种类型都不同，尽管表观分子量均匀增加.apprxeq。三万。对33例无关个体的HUE-C3bR基因分型结果表明，23例为a型，1例为b型，9例为c型。家系研究提供了2个共显性等位基因传播的证据。在正常人群中，2个等位基因似乎控制了HUE-C3bR表型的表达，并解释了这种完整的膜糖蛋白的多态性。
The human erythrocyte receptor for the major activation fragments of the 3rd and 4th components of complement [C3, C4] (HuE-C3bR) was isolated from individual donors. Erythrocytes were surface labeled with 125I and solubilized in Nonidet P-40. HuE-C3bR was purified by using C3-Sepharose affinity chromatography and analyzed by autoradiography of NaDodSO4/polyacrylamide gels. Three distinct receptor patterns were demonstrated. Type a had a single major band with MW of 190,000. Type b had a single major band with MW of 220,000, and type c had 2 major bands of MW 190,000 and 220,000. In all 3 types, a minor band accounting for < 25% of the total radioactivity was usually observed at a MW 15,000 greater than that of each major band. Identical autoradiographic patterns were obtained by affinity chromatography using methylamine-inactivated C4-Sepharose or by immunoprecipitation of solubilized membranes with a monoclonal antibody against HuE-C3bR. All 3 types were distinct after reduction and alkylation, although the apparent MW uniformly increased by .apprxeq. 30,000. Characterization of HuE-C3bR types in 33 unrelated individuals demonstrated that 23 had type a, 1 had type b, and 9 had type c. Family studies provide evidence for transmission by 2 codominant alleles. In the normal population 2 alleles appear to control expression of HuE-C3bR phenotypes and account for the polymorphism of this integral membrane glycoprotein.